Subsensitivity of P2X but not vanilloid 1 receptors in dorsal root ganglia of rats caused by cyclophosphamide cystitis

Sebestyen J. Borvendeg, Mahmoud Al-Khrasani, Patrizia Rubini, Wolfgang Fischer, Clemens Allgaier, Kerstin Wirkner, Herbert M. Himmel, Clemens Gillen, Peter Illes

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Abstract

The application of cyclophosphamide to rats was used to induce interstitial cystitis. Behavioural studies indicated a strong pain reaction that developed within 2 h and levelled off thereafter causing a constant pain during the following 18 h. Neurons prepared from L6/S1 dorsal root ganglia innervating the urinary bladder responded to the application of capsaicin or α,β-methylene ATP (α,β-meATP) with an increase of intracellular Ca2+ ([Ca2+]i). The [Ca 2+]i responses to capsaicin were identical in the dorsal root ganglion cells of cyclophosphamide- and saline-treated rats, whereas α,β-meATP induced less increase in [Ca2+]i in the cyclophosphamide-treated animals than in their saline-treated counterparts. Hence, α,β-meATP-sensitive P2X3 and/or P2X2/3 receptors of L6/S1 dorsal root ganglion neurons were functionally downregulated during subacute pain caused by experimental cystitis. In contrast, capsaicin-sensitive vanilloid 1 receptors did not react to the same procedure. Thoracal dorsal root ganglia, not innervating the urinary bladder, were also unaltered in their responsiveness to α,β-meATP by cyclophosphamide treatment.

Original languageEnglish
Pages (from-to)71-75
Number of pages5
JournalEuropean Journal of Pharmacology
Volume474
Issue number1
DOIs
Publication statusPublished - Aug 1 2003

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Keywords

  • Cyclophosphamide- induced cystitis
  • Dorsal root ganglion
  • P2X receptor
  • Vanilloid 1 receptor

ASJC Scopus subject areas

  • Pharmacology

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