Subnuclear immunofluorescence.

D. Szüts, Julian E. Sale

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Despite their relatively small size and the fact that they are naturally non-adherent, it is possible to obtain good immunofluorescence staining of subnuclear structures in DT40. This, combined with their genetic tractability, provides a powerful combination for the study of DNA replication and repair. Here we provide a general protocol for immunofluorescence of molecules such as RAD51 and phosphorylated histone H2Ax. We also present a modification to this protocol that allows visualisation of chromatin bound PCNA and hence sites of DNA synthesis.

Original languageEnglish
Pages (from-to)395-398
Number of pages4
JournalSub-Cellular Biochemistry
Volume40
Publication statusPublished - 2006

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Fluorescent Antibody Technique
DNA
Proliferating Cell Nuclear Antigen
DNA Replication
DNA Repair
Histones
Chromatin
Repair
Visualization
Staining and Labeling
Molecules

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Subnuclear immunofluorescence. / Szüts, D.; Sale, Julian E.

In: Sub-Cellular Biochemistry, Vol. 40, 2006, p. 395-398.

Research output: Contribution to journalArticle

Szüts, D & Sale, JE 2006, 'Subnuclear immunofluorescence.', Sub-Cellular Biochemistry, vol. 40, pp. 395-398.
Szüts, D. ; Sale, Julian E. / Subnuclear immunofluorescence. In: Sub-Cellular Biochemistry. 2006 ; Vol. 40. pp. 395-398.
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