Submicroscopic evidence of bacterially induced resistance in tobacco leaves

P. Ott, L. Szabó, E. Balázs, Z. Klement

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A prototrophic HR (hypersensitive reaction)-negative mutant of Pseudomonas syringae pv. phaseolicola was used to induce the early induced resistance (EIR) and the late induced resistance (LIR) in tobacco. This mutant also served as an indicator of submicroscopic host responses in non-treated, heat shocked, and dark-treated leaves. Heat shock (50°C, 20 s) and dark are known to suppress EIR and LIR, respectively. Intercellular spaces of the non-treated and heat-shocked leaves contained dividing bacteria at 6-12 hpi. At 24-48 hpi the ratio of dividing bacteria decreased with a concomitant increase of an intensely electron-dense, distorted bacterial form frequently embedded in an amorphous, electron-dense matrix. Around 12 hpi paramural papillae appeared as site-specific plant responses to the neighbouring bacterial cells. Occurrence of papillae correlated closely with the distorted, possibly damaged bacterial form. The highest number of dividing bacteria and the largest microcolonies indicated the partial lack of EIR in heat-shocked leaves, but neither the distorted bacteria nor papillae could be connected with EIR. In the dark, compared to the other two treatments, less distorted bacteria constituted looser microcolonies after 24 hpi, while papilla formation was suppressed. Thus a relationship was found between papilla formation, the distorted bacterial cells and LIR.

Original languageEnglish
Pages (from-to)265-280
Number of pages16
JournalActa Phytopathologica et Entomologica Hungarica
Volume32
Issue number3-4
Publication statusPublished - 1997

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induced resistance
tobacco
leaves
bacteria
heat
Pseudomonas syringae pv. phaseolicola
electrons
mutants
intercellular spaces
hypersensitive response
heat stress
plant response
cells

ASJC Scopus subject areas

  • Insect Science
  • Plant Science

Cite this

Submicroscopic evidence of bacterially induced resistance in tobacco leaves. / Ott, P.; Szabó, L.; Balázs, E.; Klement, Z.

In: Acta Phytopathologica et Entomologica Hungarica, Vol. 32, No. 3-4, 1997, p. 265-280.

Research output: Contribution to journalArticle

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