Subcellular distribution of biogenic monoamines in the central nervous system of Anodonta cygnea L. as revealed by density gradient centrifugation

L. Hiripi, J. Salánki, I. Z. Nagy, I. Musko

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Abstract

Differential and gradient centrifugation revealed that 90 per cent of the 5 HT, dopamine and noradrenaline in the CNS of the fresh water mussel was bound to particles; 60-70 per cent of the bound monoamines appeared in the mitochondrial and 15-20 per cent in the microsomal fraction. Spectrofluorometric assay and electron microscopic analysis of the subfractions obtained by separation of the mitochondrial fraction on sucrose density gradients showed that the nerve endings and their dense core vesicles were concentrated in fractions with high relative specific activity of the three monoamines. This supports the proposed function of these monoamines as interneuronal mediators. Osmotic shock treatment resulted in the formation of a synaptosomal subfraction of low density displaying a high relative specific activity for 5 HT. From the results obtained one cannot draw clearcut conclusions regarding the participation of subcellular particles in the storage of serotonin detectable in the perikarya by means of histochemical methods.

Original languageEnglish
Pages (from-to)791-797
Number of pages7
JournalJournal of Neurochemistry
Volume21
Issue number4
Publication statusPublished - 1973

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Biogenic Monoamines
Anodonta
Density Gradient Centrifugation
Centrifugation
Neurology
Serotonin
Central Nervous System
Nerve Endings
Bivalvia
Osmotic Pressure
Secretory Vesicles
Fresh Water
Sucrose
Assays
Dopamine
Norepinephrine
Electrons
Water

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

Cite this

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abstract = "Differential and gradient centrifugation revealed that 90 per cent of the 5 HT, dopamine and noradrenaline in the CNS of the fresh water mussel was bound to particles; 60-70 per cent of the bound monoamines appeared in the mitochondrial and 15-20 per cent in the microsomal fraction. Spectrofluorometric assay and electron microscopic analysis of the subfractions obtained by separation of the mitochondrial fraction on sucrose density gradients showed that the nerve endings and their dense core vesicles were concentrated in fractions with high relative specific activity of the three monoamines. This supports the proposed function of these monoamines as interneuronal mediators. Osmotic shock treatment resulted in the formation of a synaptosomal subfraction of low density displaying a high relative specific activity for 5 HT. From the results obtained one cannot draw clearcut conclusions regarding the participation of subcellular particles in the storage of serotonin detectable in the perikarya by means of histochemical methods.",
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T1 - Subcellular distribution of biogenic monoamines in the central nervous system of Anodonta cygnea L. as revealed by density gradient centrifugation

AU - Hiripi, L.

AU - Salánki, J.

AU - Nagy, I. Z.

AU - Musko, I.

PY - 1973

Y1 - 1973

N2 - Differential and gradient centrifugation revealed that 90 per cent of the 5 HT, dopamine and noradrenaline in the CNS of the fresh water mussel was bound to particles; 60-70 per cent of the bound monoamines appeared in the mitochondrial and 15-20 per cent in the microsomal fraction. Spectrofluorometric assay and electron microscopic analysis of the subfractions obtained by separation of the mitochondrial fraction on sucrose density gradients showed that the nerve endings and their dense core vesicles were concentrated in fractions with high relative specific activity of the three monoamines. This supports the proposed function of these monoamines as interneuronal mediators. Osmotic shock treatment resulted in the formation of a synaptosomal subfraction of low density displaying a high relative specific activity for 5 HT. From the results obtained one cannot draw clearcut conclusions regarding the participation of subcellular particles in the storage of serotonin detectable in the perikarya by means of histochemical methods.

AB - Differential and gradient centrifugation revealed that 90 per cent of the 5 HT, dopamine and noradrenaline in the CNS of the fresh water mussel was bound to particles; 60-70 per cent of the bound monoamines appeared in the mitochondrial and 15-20 per cent in the microsomal fraction. Spectrofluorometric assay and electron microscopic analysis of the subfractions obtained by separation of the mitochondrial fraction on sucrose density gradients showed that the nerve endings and their dense core vesicles were concentrated in fractions with high relative specific activity of the three monoamines. This supports the proposed function of these monoamines as interneuronal mediators. Osmotic shock treatment resulted in the formation of a synaptosomal subfraction of low density displaying a high relative specific activity for 5 HT. From the results obtained one cannot draw clearcut conclusions regarding the participation of subcellular particles in the storage of serotonin detectable in the perikarya by means of histochemical methods.

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