Studying the toxic effect of cadmium and hexavalent chromium on microbial activity of a soil and pure microbe

AAAAAA microcalorimetric method

J. Yao, F. Wang, L. Tian, Y. Zhou, H. L. Chen, K. Chen, N. Gai, R. S. Zhuang, T. Maskow, B. Ceccanti, G. Záray

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

Using TAM III multi-channel calorimetry combined with direct microorganism counting (bacteria, actinomycetes and fungi) under laboratory conditions, we determined the microbial population count, resistance and activity toward cadmium (Cd(II)) and hexavalent chromium (Cr(VI)) toxicity in soil. The thermokinetic parameters, which can represent soil microbial activity, were calculated from power-time curves of soil microbial activity obtained by microcalorimetric measurement. Simultaneous application of the two methods showed that growth rate constant (k), peak-heat output power (P max) and the number of living microorganisms decreased with increasing concentration of Cd and Cr. The accumulation of Cr on E. coli was conducted by HPLC-ICP-MS. Cr6+ accumulation by Escherichia coli was increased steadily with increasing Cr6+ concentration. The results revealed that the change in some thermo-kinetic parameters could have good corresponding relationship with metal accumulation. Our work also suggests that microcalorimetry is a fast, simple, more sensitive, on-line and in vitro method that can be easily performed to study the toxicity of different species of heavy metals on microorganism compared to other biological methods, and can combine with other analytic methods to study the interaction mechanism between environmental toxicants and microbes.

Original languageEnglish
Pages (from-to)517-524
Number of pages8
JournalJournal of Thermal Analysis and Calorimetry
Volume95
Issue number2
DOIs
Publication statusPublished - Feb 2009

Fingerprint

Poisons
microorganisms
Cadmium
Microorganisms
cadmium
chromium
soils
Soils
Escherichia coli
Toxicity
toxicity
actinomycetes
Calorimetry
Heavy Metals
Fungi
Kinetic parameters
fungi
inductively coupled plasma mass spectrometry
Rate constants
Bacteria

Keywords

  • Cadmium
  • Escherichia coli
  • Hexavalent chromium
  • HPLC-ICP-MS
  • Microcalorimetry
  • Soil microbial activity

ASJC Scopus subject areas

  • Physical and Theoretical Chemistry
  • Condensed Matter Physics

Cite this

Studying the toxic effect of cadmium and hexavalent chromium on microbial activity of a soil and pure microbe : AAAAAA microcalorimetric method. / Yao, J.; Wang, F.; Tian, L.; Zhou, Y.; Chen, H. L.; Chen, K.; Gai, N.; Zhuang, R. S.; Maskow, T.; Ceccanti, B.; Záray, G.

In: Journal of Thermal Analysis and Calorimetry, Vol. 95, No. 2, 02.2009, p. 517-524.

Research output: Contribution to journalArticle

Yao, J. ; Wang, F. ; Tian, L. ; Zhou, Y. ; Chen, H. L. ; Chen, K. ; Gai, N. ; Zhuang, R. S. ; Maskow, T. ; Ceccanti, B. ; Záray, G. / Studying the toxic effect of cadmium and hexavalent chromium on microbial activity of a soil and pure microbe : AAAAAA microcalorimetric method. In: Journal of Thermal Analysis and Calorimetry. 2009 ; Vol. 95, No. 2. pp. 517-524.
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