Study on the glutathione metabolism of the filamentous fungus aspergillus nidulans

Fruzsina Bakti, Anita Király, Erzsébet Orosz, Márton Miskei, T. Emri, E. Leiter, I. Pócsi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Yeast protein sequence-based homology search for glutathione (GSH) metabolic enzymes and GSH transporters demonstrated that Aspergillus nidulans has a robust GSH uptake and metabolic system with several paralogous genes. In wet laboratory experiments, two key genes of GSH metabolism, gcsA, and glrA, encoding γ-L-glutamyl-L-cysteine synthetase and glutathione reductase, respectively, were deleted. The gene gcsA was essential, and the δgcsA mutant required GSH supplementation at considerably higher concentration than the Saccharomyces cerevisiae gsh1 mutant (8-10 mmol l-1 vs. 0.5 μmol l-1). In addition to some functions known previously, both genes were important in the germination of conidiospores, and both gene deletion strains required the addition of extra GSH to reach wild-type germination rates in liquid cultures. Nevertheless, the supplementation of cultures with 10 mmol l-1 GSH was toxic for the control and δglrA strains especially during vegetative growth, which should be considered in future development of high GSHproducer fungal strains. Importantly, the δglrA strain was characterized by increased sensitivity toward a wide spectrum of osmotic, cell wall integrity and antimycotic stress conditions in addition to previously reported temperature and oxidative stress sensitivities. These novel phenotypes underline the distinguished functions of GSH and GSH metabolic enzymes in the stress responses of fungi.

Original languageEnglish
Pages (from-to)255-272
Number of pages18
JournalActa Microbiologica et Immunologica Hungarica
Volume64
Issue number3
DOIs
Publication statusPublished - Sep 1 2017

Fingerprint

Aspergillus nidulans
Glutathione
Fungi
Germination
Genes
Amino Acid Sequence Homology
Fungal Proteins
Glutathione Reductase
Poisons
Gene Deletion
Enzymes
Ligases
Cell Wall
Cysteine
Saccharomyces cerevisiae
Oxidative Stress
Phenotype
Temperature
Growth

Keywords

  • Aspergillus nidulans
  • Environmental stress
  • Glutathione
  • Glutathione metabolism
  • In silico analysis

ASJC Scopus subject areas

  • Immunology and Microbiology(all)

Cite this

Study on the glutathione metabolism of the filamentous fungus aspergillus nidulans. / Bakti, Fruzsina; Király, Anita; Orosz, Erzsébet; Miskei, Márton; Emri, T.; Leiter, E.; Pócsi, I.

In: Acta Microbiologica et Immunologica Hungarica, Vol. 64, No. 3, 01.09.2017, p. 255-272.

Research output: Contribution to journalArticle

Bakti, Fruzsina ; Király, Anita ; Orosz, Erzsébet ; Miskei, Márton ; Emri, T. ; Leiter, E. ; Pócsi, I. / Study on the glutathione metabolism of the filamentous fungus aspergillus nidulans. In: Acta Microbiologica et Immunologica Hungarica. 2017 ; Vol. 64, No. 3. pp. 255-272.
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