Study on heterogeneity of β-glucosidase from Aspergillus species by using counter-current distribution

Anikó Brumbauer, Göte Johansson, Kati Réczey

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

β-Glucosidase plays a key role in cellulose degradation by cleaving its cellobiose units to glucose. The heterogeneity of the enzyme has been studied earlier by isoelectric focusing and several isoforms were found in the culture filtrate of Trichoderma and Aspergillus species. An aqueous two-phase system is an alternative fractionation method in which proteins are separated according to their net charge and surface properties. A dextran 500- poly(ethylene glycol) 8000 system was used to study the distribution of β- glucosidase of different origins. To investigate the heterogeneity of enzymes 56- and 145-step thin-layer counter-current distributions were carried out. β-Glucosidase produced by Aspergillus niger and Aspergillus carbonarius has been shown to consist of at least three isoenzymes by applying this method. The three forms of A. niger show the corresponding partition ratios with G values of 0.13, 0.22 and 0.31; and A. carbonarius 0.14, 0.22 and 0.36, respectively. However, the relative amount of these isoforms differs strongly between the two microorganisms. (C) 2000 Elsevier Science B.V.

Original languageEnglish
Pages (from-to)247-254
Number of pages8
JournalJournal of Chromatography B: Biomedical Sciences and Applications
Volume743
Issue number1-2
DOIs
Publication statusPublished - Jun 23 2000

Keywords

  • Aqueous two-phase systems
  • Aspergillus
  • Counter-current distribution
  • Enzymes
  • Partitioning
  • β-Glucosidase

ASJC Scopus subject areas

  • Chemistry(all)

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