Study of the rhizobacterium Azospirillum brasilense Sp245 using Mössbauer spectroscopy with a high velocity resolution: Implication for the analysis of ferritin-like iron cores

I. V. Alenkina, M. I. Oshtrakh, A. V. Tugarova, B. Biró, V. A. Semionkin, A. A. Kamnev

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Abstract

The results of a comparative study of two samples of the rhizobacterium Azospirillum brasilense (strain Sp245) prepared in different conditions and of human liver ferritin using Mössbauer spectroscopy with a high velocity resolution demonstrated the presence of ferritin-like iron (i.e. iron similar to that found in ferritin-like proteins) in the bacterium. Mössbauer spectra of these samples were fitted in two ways: as a rough approximation using a one quadrupole doublet fit (the homogeneous iron core model) and using a superposition of quadrupole doublets (the heterogeneous iron core model). Both results demonstrated differences in the Mössbauer parameters for mammalian ferritin and for bacterial ferritin-like iron. Moreover, some differences in the Mössbauer parameters were observed between the two samples of A. brasilense Sp245 related to the differences in their preparation conditions.

Original languageEnglish
Pages (from-to)181-186
Number of pages6
JournalJournal of Molecular Structure
Volume1073
Issue numberC
DOIs
Publication statusPublished - Sep 5 2014

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Keywords

  • Azospirillum brasilense Sp245
  • Ferritin-like iron core
  • Mössbauer spectroscopy
  • Rhizobacterium

ASJC Scopus subject areas

  • Analytical Chemistry
  • Spectroscopy
  • Organic Chemistry
  • Inorganic Chemistry

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