Study of the Retention Parameters of barbituric acid derivatives in reversed phase HPLC by using quantitative structure-retention relationships

A. Jakab, G. Schubert, M. Prodan, E. Forgács

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Retention parameters of 45 different barbituric acid derivatives were determined on an amide embedded RP silica column (Discovery RP-AmideC16) using non-buffered methanol-water mixtures as eluents. Linear correlations were calculated between the logk values and the methanol concentration in the eluent. The intercept (logk0) and slope (b) values of the line, the combined retention parameter (logk0/b), asymmetry factor (AF5) and theoretical plate (N) value of each barbituric acid derivative were correlated with different conventional and quantum chemical structural descriptors using Quantitative Structure-Retention Relationship (QSRR). The SRA (stepwise regression analysis) results indicated that the retention is mainly governed by the steric and the lipophilicity properties of the barbituric acid derivatives in water-methanol eluent mixtures.

Original languageEnglish
Pages (from-to)S55-S59
JournalChromatographia
Volume56
Issue numberSUPPL.
DOIs
Publication statusPublished - Jan 1 2002

Keywords

  • Amide embedded reversed phase silica column
  • Barbituric acid derivatives
  • Column liquid chromatography
  • QSRR

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Clinical Biochemistry
  • Organic Chemistry

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