Study of the LMW glutenin composition of some old Hungarian wheat cultivars using capillary electrophoresis

M. Rakszegi, É Scholz, M. Kárpáti, K. Ganzler, R. Lásztity, Z. Bedő

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The LMW glutenin composition of nine old Hungarian wheats (several lines per variety), and one Canadian wheat was studied using one-step, one-dimensional SDS-PAGE (Gupta and MacRitchie, 1991), after which a method was developed for the determination of wheat LMW glutenin patterns using SDS capillary electrophoresis (CE). In both cases the samples were prepared as described by Gupta and MacRitchie (1991), with some modifications in the case of CE. Both methods were used to analyse the homogeneity of the varieties, and the electropherograms obtained with the two methods were compared. It was found that the new method based on CE was suitable for the demonstration of differences and similarities between the wheat LMW glutenins. The method can be used to reveal differences not only between varieties (e.g. between the Hungarian variety Bánkúti 1205 and the Canadian variety Marquis), but also between the genotypes making up a variety population (e.g. the sublines of Bánkúti 1205). The results indicate that CE is a satisfactory alternative to the traditional PAGE method for the determination of the LMW glutenin subunits of wheat varieties.

Original languageEnglish
Pages (from-to)417-424
Number of pages8
JournalCereal Research Communications
Volume28
Issue number4
Publication statusPublished - 2000

Fingerprint

capillary electrophoresis
glutenins
Capillary Electrophoresis
Triticum
wheat
cultivars
polyacrylamide gel electrophoresis
methodology
glutenin
Polyacrylamide Gel Electrophoresis
Genotype
genotype
Population

Keywords

  • Capillary electrophoresis
  • LMW glutenin
  • SDS-PAGE
  • Wheat

ASJC Scopus subject areas

  • Agronomy and Crop Science

Cite this

Study of the LMW glutenin composition of some old Hungarian wheat cultivars using capillary electrophoresis. / Rakszegi, M.; Scholz, É; Kárpáti, M.; Ganzler, K.; Lásztity, R.; Bedő, Z.

In: Cereal Research Communications, Vol. 28, No. 4, 2000, p. 417-424.

Research output: Contribution to journalArticle

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AU - Ganzler, K.

AU - Lásztity, R.

AU - Bedő, Z.

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