Study of the effect of simulated space environment on nucleoprotein and DNA thin films

A. Fekete, K. Módos, M. Hegedüs, G. Rontó, G. Kovács, A. Bérces, G. Kargl, N. I. Kömle, H. Lammer

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy, To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (λ= 254 nm) and high vacuum (10 -5 mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.

Original languageEnglish
Title of host publicationEuropean Space Agency, (Special Publication) ESA SP
EditorsH. Lacoste
Pages67-70
Number of pages4
Edition518
Publication statusPublished - Sep 2002
EventProceedings of the Second European Workshop on Exo-Astrobiology - Graz, Austria
Duration: Sep 16 2002Sep 19 2002

Other

OtherProceedings of the Second European Workshop on Exo-Astrobiology
CountryAustria
CityGraz
Period9/16/029/19/02

Fingerprint

DNA
Bacteriophages
Thin films
Nucleic acids
Radiation damage
Ultraviolet spectroscopy
Ultraviolet radiation
Hydration
Conformations
Absorption spectra
Microscopic examination
Vacuum
Radiation
Experiments

ASJC Scopus subject areas

  • Aerospace Engineering

Cite this

Fekete, A., Módos, K., Hegedüs, M., Rontó, G., Kovács, G., Bérces, A., ... Lammer, H. (2002). Study of the effect of simulated space environment on nucleoprotein and DNA thin films. In H. Lacoste (Ed.), European Space Agency, (Special Publication) ESA SP (518 ed., pp. 67-70)

Study of the effect of simulated space environment on nucleoprotein and DNA thin films. / Fekete, A.; Módos, K.; Hegedüs, M.; Rontó, G.; Kovács, G.; Bérces, A.; Kargl, G.; Kömle, N. I.; Lammer, H.

European Space Agency, (Special Publication) ESA SP. ed. / H. Lacoste. 518. ed. 2002. p. 67-70.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Fekete, A, Módos, K, Hegedüs, M, Rontó, G, Kovács, G, Bérces, A, Kargl, G, Kömle, NI & Lammer, H 2002, Study of the effect of simulated space environment on nucleoprotein and DNA thin films. in H Lacoste (ed.), European Space Agency, (Special Publication) ESA SP. 518 edn, pp. 67-70, Proceedings of the Second European Workshop on Exo-Astrobiology, Graz, Austria, 9/16/02.
Fekete A, Módos K, Hegedüs M, Rontó G, Kovács G, Bérces A et al. Study of the effect of simulated space environment on nucleoprotein and DNA thin films. In Lacoste H, editor, European Space Agency, (Special Publication) ESA SP. 518 ed. 2002. p. 67-70
Fekete, A. ; Módos, K. ; Hegedüs, M. ; Rontó, G. ; Kovács, G. ; Bérces, A. ; Kargl, G. ; Kömle, N. I. ; Lammer, H. / Study of the effect of simulated space environment on nucleoprotein and DNA thin films. European Space Agency, (Special Publication) ESA SP. editor / H. Lacoste. 518. ed. 2002. pp. 67-70
@inproceedings{3486544e31e14844ac9fb939d02d6712,
title = "Study of the effect of simulated space environment on nucleoprotein and DNA thin films",
abstract = "The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy, To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (λ= 254 nm) and high vacuum (10 -5 mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.",
author = "A. Fekete and K. M{\'o}dos and M. Heged{\"u}s and G. Ront{\'o} and G. Kov{\'a}cs and A. B{\'e}rces and G. Kargl and K{\"o}mle, {N. I.} and H. Lammer",
year = "2002",
month = "9",
language = "English",
pages = "67--70",
editor = "H. Lacoste",
booktitle = "European Space Agency, (Special Publication) ESA SP",
edition = "518",

}

TY - GEN

T1 - Study of the effect of simulated space environment on nucleoprotein and DNA thin films

AU - Fekete, A.

AU - Módos, K.

AU - Hegedüs, M.

AU - Rontó, G.

AU - Kovács, G.

AU - Bérces, A.

AU - Kargl, G.

AU - Kömle, N. I.

AU - Lammer, H.

PY - 2002/9

Y1 - 2002/9

N2 - The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy, To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (λ= 254 nm) and high vacuum (10 -5 mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.

AB - The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy, To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (λ= 254 nm) and high vacuum (10 -5 mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.

UR - http://www.scopus.com/inward/record.url?scp=0036766073&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0036766073&partnerID=8YFLogxK

M3 - Conference contribution

SP - 67

EP - 70

BT - European Space Agency, (Special Publication) ESA SP

A2 - Lacoste, H.

ER -