Study of the effect of simulated space environment on nucleoprotein and DNA thin films

A. Fekete, K. Módos, M. Hegedüs, Gy Rontó, G. Kovács, A. Bérces, G. Kargl, N. I. Kömle, H. Lammer

Research output: Contribution to journalConference article

Abstract

The main goal of PUR experiment (phage and uracil response) is to examine and quantify the effect of specific space conditions on nucleic acid models. To achieve this an improved method was elaborated for the preparation of DNA and bacteriophage thin films. The homogeneity of the films was controlled by UV spectroscopy and microscopy, To provide experimental evidence for the hypothesis that interplanetary transfer of life is possible, phage T7 and isolated T7 DNA thin films have been exposed to selected space conditions: intense UVC radiation (λ= 254 nm) and high vacuum (10 -5 mbar). The effects of DNA hydration, conformation and packing on UV radiation damage were examined. Characteristic changes in the absorption spectrum, in the electrophoretic pattern of DNA and the decrease of the amount of PCR products have been detected indicating the photodamage of isolated and intraphage DNA.

Original languageEnglish
Pages (from-to)67-70
Number of pages4
JournalEuropean Space Agency, (Special Publication) ESA SP
Issue number518
Publication statusPublished - Sep 1 2002
EventProceedings of the Second European Workshop on Exo-Astrobiology - Graz, Austria
Duration: Sep 16 2002Sep 19 2002

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ASJC Scopus subject areas

  • Aerospace Engineering
  • Space and Planetary Science

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