Study of the determination of acetylcholine after enzymatic hydrolysis by triangle programmed coulometric flow titration

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Abstract

A new method for the determination of acetylcholine is introduced and studied. The method uses enzymatic hydrolysis of acetylcholine carried out in a flow-through analytical reactor column, while for the detection triangle programmed coulometric flow titration is employed. The flow-through coulometric titration system and the preparation of the enzyme reactor are described. The operation conditions for the hydrolysis and for the titration were optimized. The flow-through coulometric titration technique offers a better precision for the determination of acetylcholine in small concentration ranges than methods based on the detection of pH change. Working conditions of the system can be easily adjusted for different sample concentration ranges. The limit of detection for the determination of acetylcholine was found to be 8 x 10-5 M. The method described could be advantageously used for indirect determination of acetylcholinesterase inhibitors.

Original languageEnglish
Pages (from-to)1021-1031
Number of pages11
JournalTalanta
Volume47
Issue number4
DOIs
Publication statusPublished - Sep 28 1998

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Keywords

  • Acetylcholine
  • Coulometric titration
  • Enzyme reactor
  • Flow analysis

ASJC Scopus subject areas

  • Analytical Chemistry

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