Study of the competitive binding of TBA and CP ions on C18 surface

G. Szász, Zs Budvári-Bárány, K. Gyimesi-Forrás

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

The adsorption behavior of tetrabutylammonium (TBA) and cetylpyridinium (CP) cations was studied on Hypersil 5-ODS sorbent. After establishing that CP has about 5-fold stronger affinity to the C18 surface than the structurally different, symmetric, spherical molecule of TBA, the adsorption from their common solution was also examined. The data of 'competitive binding' seem to show that TBA should have certain selective binding sites on the C18 surface from which it can not be displaced by the CP, even in the case where the latter is present in a 4-fold excess in the mobile phase. The maximal coverage was found to be 52.9% (CP) and 12% (TBA), i.e., every second C18 group is covered by CP cations and only 1/8th of C18 groups may interact with TBA. The accessible, proton releasing silanol content, related to the total C18 content of the column, was found to be very low.

Original languageEnglish
Pages (from-to)3211-3220
Number of pages10
JournalJournal of Liquid Chromatography and Related Technologies
Volume19
Issue number19
Publication statusPublished - 1996

Fingerprint

Cetylpyridinium
Competitive Binding
Ions
Adsorption
Cations
Sorbents
Protons
Binding Sites
tetrabutylammonium
Molecules

ASJC Scopus subject areas

  • Clinical Biochemistry
  • Analytical Chemistry

Cite this

Study of the competitive binding of TBA and CP ions on C18 surface. / Szász, G.; Budvári-Bárány, Zs; Gyimesi-Forrás, K.

In: Journal of Liquid Chromatography and Related Technologies, Vol. 19, No. 19, 1996, p. 3211-3220.

Research output: Contribution to journalArticle

Szász, G. ; Budvári-Bárány, Zs ; Gyimesi-Forrás, K. / Study of the competitive binding of TBA and CP ions on C18 surface. In: Journal of Liquid Chromatography and Related Technologies. 1996 ; Vol. 19, No. 19. pp. 3211-3220.
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