Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation

Cristina A. Dehelean, Codruţa Şoica, Ionuţ Ledeţi, Mihaela Aluaş, Istvan Zupko, Atena Gǎluşcan, Simona Cinta-Pinzaru, Melania Munteanu

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Abstract

Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.

Original languageEnglish
Article number137
JournalChemistry Central Journal
Volume6
Issue number1
DOIs
Publication statusPublished - Nov 19 2012

Keywords

  • 13C NMR
  • Anticancer
  • Betulin
  • Birch extract
  • Raman
  • SERS

ASJC Scopus subject areas

  • Chemistry(all)

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    Dehelean, C. A., Şoica, C., Ledeţi, I., Aluaş, M., Zupko, I., Gǎluşcan, A., Cinta-Pinzaru, S., & Munteanu, M. (2012). Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation. Chemistry Central Journal, 6(1), [137]. https://doi.org/10.1186/1752-153X-6-137