Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation

Cristina A. Dehelean, Codruţa Şoica, Ionuţ Ledeţi, Mihaela Aluaş, I. Zupkó, Atena Gǎluşcan, Simona Cinta-Pinzaru, Melania Munteanu

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.

Original languageEnglish
Article number137
JournalChemistry Central Journal
Volume6
Issue number1
DOIs
Publication statusPublished - Nov 19 2012

Fingerprint

Anti-Inflammatory Agents
Pentacyclic Triterpenes
Cells
Antineoplastic Agents
Nuclear magnetic resonance spectroscopy
Assays
Skin
Chemical analysis
betulin
betulinic acid

Keywords

  • 13C NMR
  • Anticancer
  • Betulin
  • Birch extract
  • Raman
  • SERS

ASJC Scopus subject areas

  • Chemistry(all)

Cite this

Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation. / Dehelean, Cristina A.; Şoica, Codruţa; Ledeţi, Ionuţ; Aluaş, Mihaela; Zupkó, I.; Gǎluşcan, Atena; Cinta-Pinzaru, Simona; Munteanu, Melania.

In: Chemistry Central Journal, Vol. 6, No. 1, 137, 19.11.2012.

Research output: Contribution to journalArticle

Dehelean, CA, Şoica, C, Ledeţi, I, Aluaş, M, Zupkó, I, Gǎluşcan, A, Cinta-Pinzaru, S & Munteanu, M 2012, 'Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation', Chemistry Central Journal, vol. 6, no. 1, 137. https://doi.org/10.1186/1752-153X-6-137
Dehelean, Cristina A. ; Şoica, Codruţa ; Ledeţi, Ionuţ ; Aluaş, Mihaela ; Zupkó, I. ; Gǎluşcan, Atena ; Cinta-Pinzaru, Simona ; Munteanu, Melania. / Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation. In: Chemistry Central Journal. 2012 ; Vol. 6, No. 1.
@article{91a3c789681f4a78bbf08b4592b71b7e,
title = "Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation",
abstract = "Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90{\%}). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.",
keywords = "13C NMR, Anticancer, Betulin, Birch extract, Raman, SERS",
author = "Dehelean, {Cristina A.} and Codruţa Şoica and Ionuţ Ledeţi and Mihaela Aluaş and I. Zupk{\'o} and Atena Gǎluşcan and Simona Cinta-Pinzaru and Melania Munteanu",
year = "2012",
month = "11",
day = "19",
doi = "10.1186/1752-153X-6-137",
language = "English",
volume = "6",
journal = "Chemistry Central Journal",
issn = "1752-153X",
publisher = "Chemistry Central",
number = "1",

}

TY - JOUR

T1 - Study of the betulin enriched birch bark extracts effects on human carcinoma cells and ear inflammation

AU - Dehelean, Cristina A.

AU - Şoica, Codruţa

AU - Ledeţi, Ionuţ

AU - Aluaş, Mihaela

AU - Zupkó, I.

AU - Gǎluşcan, Atena

AU - Cinta-Pinzaru, Simona

AU - Munteanu, Melania

PY - 2012/11/19

Y1 - 2012/11/19

N2 - Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.

AB - Background: Pentacyclic triterpenes, mainly betulin and betulinic acid, are valuable anticancer agents found in the bark of birch tree. This study evaluates birch bark extracts for the active principles composition.Results: New improved extraction methods were applied on the bark of Betula pendula in order to reach the maximum content in active principles. Extracts were analyzed by HPLC-MS, Raman, SERS and 13C NMR spectroscopy which revealed a very high yield of betulin (over 90%). Growth inhibiting effects were measured in vitro on four malignant human cell lines: A431 (skin epidermoid carcinoma), A2780 (ovarian carcinoma), HeLa (cervix adenocarcinoma) and MCF7 (breast adenocarcinoma), by means of MTT assay. All of the prepared bark extracts exerted a pronounced antiproliferative effect against human cancer cell lines. In vivo studies involved the anti-inflammatory effect of birch extracts on TPA-induced model of inflammation in mice.Conclusions: The research revealed the efficacy of the extraction procedures as well as the antiproliferative and anti-inflammatory effects of birch extracts.

KW - 13C NMR

KW - Anticancer

KW - Betulin

KW - Birch extract

KW - Raman

KW - SERS

UR - http://www.scopus.com/inward/record.url?scp=84869116273&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84869116273&partnerID=8YFLogxK

U2 - 10.1186/1752-153X-6-137

DO - 10.1186/1752-153X-6-137

M3 - Article

VL - 6

JO - Chemistry Central Journal

JF - Chemistry Central Journal

SN - 1752-153X

IS - 1

M1 - 137

ER -