Study of solvent-protein coupling effects by neutron scattering

B. Varga, F. Migliardo, E. Takacs, B. Vértessy, Salvatore Magazù, M. T F Telling

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

The present work aims to characterize the dynamical behavior of proteins immersed in bio-preserving liquids and glasses. For this purpose, the protein dUTPase was chosen, while the selected solvents were glycerol, a triol, and some homologous disaccharides, i.e., trehalose, maltose, and sucrose, which are known to be very effective bio-preserving agents. The results highlight that the disaccharides show a slowing down effect on the water dynamics, which is stronger for trehalose than in the case of the other disaccharides. Furthermore, a characterization of the medium which hosts the protein is performed by using an operative definition of fragility based on the mean square displacement extracted by elastic incoherent neutron scattering, which is directly connected to Angell's kinetic fragility based on the viscosity. Finally, a study of the dynamics of the protein sequestered within the solvents is performed. The result shows that the protein dynamics is coupled with that of the surrounding matrix.

Original languageEnglish
Pages (from-to)207-220
Number of pages14
JournalJournal of Biological Physics
Volume36
Issue number2
DOIs
Publication statusPublished - Mar 2010

Fingerprint

Neutrons
neutron scattering
Disaccharides
proteins
Trehalose
Proteins
preserving
triols
incoherent scattering
Maltose
sucrose
glycerols
Viscosity
Glycerol
Glass
Sucrose
viscosity
Water
glass
kinetics

Keywords

  • Bioprotection
  • Elastic neutron scattering
  • Protein
  • Quasi-elastic neutron scattering

ASJC Scopus subject areas

  • Biophysics
  • Cell Biology
  • Molecular Biology
  • Atomic and Molecular Physics, and Optics

Cite this

Varga, B., Migliardo, F., Takacs, E., Vértessy, B., Magazù, S., & Telling, M. T. F. (2010). Study of solvent-protein coupling effects by neutron scattering. Journal of Biological Physics, 36(2), 207-220. https://doi.org/10.1007/s10867-009-9177-5

Study of solvent-protein coupling effects by neutron scattering. / Varga, B.; Migliardo, F.; Takacs, E.; Vértessy, B.; Magazù, Salvatore; Telling, M. T F.

In: Journal of Biological Physics, Vol. 36, No. 2, 03.2010, p. 207-220.

Research output: Contribution to journalArticle

Varga, B, Migliardo, F, Takacs, E, Vértessy, B, Magazù, S & Telling, MTF 2010, 'Study of solvent-protein coupling effects by neutron scattering', Journal of Biological Physics, vol. 36, no. 2, pp. 207-220. https://doi.org/10.1007/s10867-009-9177-5
Varga, B. ; Migliardo, F. ; Takacs, E. ; Vértessy, B. ; Magazù, Salvatore ; Telling, M. T F. / Study of solvent-protein coupling effects by neutron scattering. In: Journal of Biological Physics. 2010 ; Vol. 36, No. 2. pp. 207-220.
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