Study of reactive oxygen or/and nitrogen species binding processes on E. coli bacteria with mass spectrometry isotopic nanoimaging

David Duday, Franck Clément, Elodie Lecoq, Christian Penny, Jean Nicolas Audinot, Thierry Belmonte, Kinga Kutasi, Henry Michel Cauchie, Patrick Choquet

Research output: Contribution to journalArticle

15 Citations (Scopus)

Abstract

Today, it is necessary to better understand the role of reactive species in plasmas on biological objects in order to use plasma processes for biomedical applications. Even when well-known plasma set-ups producing one main reactive species in a controlled environment are used, it is still difficult to understand reactive species interaction processes with biological systems. In this study, nanoimaging with the most sensitive mass spectrometry technology, and the use of isotopic 15N2 or/and 18O 2 containing-Ar gas mixtures to produce the plasma is proposed in order to obtain isotopic and elemental data on microwave late afterglow treated Escherichia coli bacteria. By this way, new data dealing with reactive species binding processes on plasma treated bacteria by using NanoSIMS50 mass spectrometer are obtained. The binding of ROS and RNS on Escherichia coli in microwave late afterglow is followed by using isotopic nanoimaging with an ultrasensitive mass spectrometer. Relationships between plasma composition, plasma treatment duration and the binding of reactive species on and inside bacteria are studied. Atomic nitrogen and atomic oxygen rapidly bind on the bacterial membrane and also rapidly diffuses inside bacteria to react with intracellular material.

Original languageEnglish
Pages (from-to)864-879
Number of pages16
JournalPlasma Processes and Polymers
Volume10
Issue number10
DOIs
Publication statusPublished - Aug 1 2013

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Keywords

  • biomedical applications of plasmas
  • isotopic imaging
  • mass spectrometry
  • microwave discharges
  • plasma treatment

ASJC Scopus subject areas

  • Condensed Matter Physics
  • Polymers and Plastics

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