Study of Karolinska Institutet and Washington University polyomaviruses in tonsil, adenoid, throat swab and middle ear fluid samples

Eszter Csoma, György Lengyel, K. Bányai, Péter Takács, Noel Ánosi, Szilvia Marton, Mária Mátyus, Erika Pászti, L. Gergely, Attila Szucs

Research output: Contribution to journalArticle

Abstract

Aim: To study prevalence of Karolinska Institutet (KI) and Washington University (WU) polyomavirus (PyV) in 100 tonsils, 100 adenoids, 146 throat swab and 15 middle ear fluid samples collected from 146 patients (120 children and 26 adults), to analyze the sequence of noncoding control region (NCCR) and complete WUPyV genomes. Materials & methods: Viruses were detected by quantitative real-time PCR. The NCCRs and WUPyV genomes were sequenced and analyzed. Results: The frequency of WUPyV and KIPyV DNA was 27 and 11% in adenoids, 4 and 3% in tonsils, 4.1 and 1.4% in throat swab samples, respectively. The WUPyV DNA was detected in one middle ear fluid sample as well. The WUPyV NCCRs showed mutations which may alter the putative transcription factor binding sites. Phylogenetic analysis revealed three clades of WUPyV. Conclusion: Tonsils and adenoids might be site of virus replication and/or persistence, and WUPyV may invade into the middle ear.

Original languageEnglish
Pages (from-to)1719-1730
Number of pages12
JournalFuture Microbiology
Volume13
Issue number16
DOIs
Publication statusPublished - Dec 1 2018

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Adenoids
Polyomavirus
Palatine Tonsil
Middle Ear
Pharynx
Genome
DNA
Virus Replication
Sequence Analysis
Real-Time Polymerase Chain Reaction
Transcription Factors
Cross-Sectional Studies
Binding Sites
Viruses
Mutation

Keywords

  • adenoid
  • complete genome and NCCR sequence
  • KIPyV
  • tonsil
  • WUPyV prevalence

ASJC Scopus subject areas

  • Microbiology
  • Microbiology (medical)

Cite this

Study of Karolinska Institutet and Washington University polyomaviruses in tonsil, adenoid, throat swab and middle ear fluid samples. / Csoma, Eszter; Lengyel, György; Bányai, K.; Takács, Péter; Ánosi, Noel; Marton, Szilvia; Mátyus, Mária; Pászti, Erika; Gergely, L.; Szucs, Attila.

In: Future Microbiology, Vol. 13, No. 16, 01.12.2018, p. 1719-1730.

Research output: Contribution to journalArticle

Csoma, Eszter ; Lengyel, György ; Bányai, K. ; Takács, Péter ; Ánosi, Noel ; Marton, Szilvia ; Mátyus, Mária ; Pászti, Erika ; Gergely, L. ; Szucs, Attila. / Study of Karolinska Institutet and Washington University polyomaviruses in tonsil, adenoid, throat swab and middle ear fluid samples. In: Future Microbiology. 2018 ; Vol. 13, No. 16. pp. 1719-1730.
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