Study of interaction of iron and lead during their uptake process in wheat roots by total-reflection X-ray fluorescence spectrometry

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Microwave assisted acidic digestion and total-reflection X-ray fluorescence spectrometry (TXRF) was used for the determination of lead and iron in wheat roots cultured in CaSO4 solution, and treated with Pb(NO3)2 and Fe(III)-citrate or Fe(III)-EDTA under controlled conditions, respectively. It was established that lead has a stimulation effect on the iron uptake in the presence of Fe(III)-citrate. The lead uptake, however, is hardly influenced by iron independently from the complex forming agents applied. To check the stability of the accumulated iron and lead constituents, some of the roots were washed with various solutions and the removable iron and lead were also measured by TXRF. These experiments indicate that the presence of lead results in higher stability of iron constituents in the root; however, iron does not have any effect on the lead constituents, the stabilities of which increase in the order Pb-citrate < Pb-(cell wall) < Pb-EDTA.

Original languageEnglish
Pages (from-to)1027-1032
Number of pages6
JournalSpectrochimica Acta - Part B Atomic Spectroscopy
Issue number7
Publication statusPublished - Jul 1 1997



  • Iron
  • Lead
  • TXRF
  • Uptake
  • Wheat

ASJC Scopus subject areas

  • Analytical Chemistry
  • Atomic and Molecular Physics, and Optics
  • Instrumentation
  • Spectroscopy

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