Study of equilibrium solubility measurement by saturation shake-flask method using hydrochlorothiazide as model compound

Edit Baka, John E A Comer, K. Takács-Novák

Research output: Contribution to journalArticle

167 Citations (Scopus)

Abstract

The experimental conditions that affect equilibrium solubility values measured by the classical saturation shake-flask method have been examined, using hydrochlorothiazide as a model compound. Modifications in temperature, sedimentation time, composition of aqueous buffer and the technique of separation of solid and liquid phases were all found to influence the equilibrium solubility results strongly. However, variations in the amount of solid excess and stirring time were found to have less influence. In the light of these observations, a new, shorter protocol has been developed for measurements of equilibrium solubility, together with recommendations for good analytical practice. The equilibrium solubilities of five other drugs were measured to verify the new protocol.

Original languageEnglish
Pages (from-to)335-341
Number of pages7
JournalJournal of Pharmaceutical and Biomedical Analysis
Volume46
Issue number2
DOIs
Publication statusPublished - Jan 22 2008

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Saturation (materials composition)
Hydrochlorothiazide
Solubility
Sedimentation
Buffers
Temperature
Liquids
Chemical analysis
Pharmaceutical Preparations

Keywords

  • Hydrochlorothiazide
  • Saturation shake-flask method
  • Solubility

ASJC Scopus subject areas

  • Analytical Chemistry
  • Pharmaceutical Science

Cite this

Study of equilibrium solubility measurement by saturation shake-flask method using hydrochlorothiazide as model compound. / Baka, Edit; Comer, John E A; Takács-Novák, K.

In: Journal of Pharmaceutical and Biomedical Analysis, Vol. 46, No. 2, 22.01.2008, p. 335-341.

Research output: Contribution to journalArticle

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