Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides

Andrea Vass, José Robles-Molina, Patricia Pérez-Ortega, Bienvenida Gilbert-López, M. Dernovics, Antonio Molina-Díaz, Juan F. García-Reyes

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The aim of the study was to evaluate the performance of different chromatographic approaches for the liquid chromatography/mass spectrometry (LC-MS(/MS)) determination of 24 highly polar pesticides. The studied compounds, which are in most cases unsuitable for conventional LC-MS(/MS) multiresidue methods were tested with nine different chromatographic conditions, including two different hydrophilic interaction liquid chromatography (HILIC) columns, two zwitterionic-type mixed-mode columns, three normal-phase columns operated in HILIC-mode (bare silica and two silica-based chemically bonded columns (cyano and amino)), and two standard reversed-phase C18 columns. Different sets of chromatographic parameters in positive (for 17 analytes) and negative ionization modes (for nine analytes) were examined. In order to compare the different approaches, a semi-quantitative classification was proposed, calculated as the percentage of an empirical performance value, which consisted of three main features: (i) capacity factor (k) to characterize analyte separation from the void, (ii) relative response factor, and (iii) peak shape based on analytes’ peak width. While no single method was able to provide appropriate detection of all the 24 studied species in a single run, the best suited approach for the compounds ionized in positive mode was based on a UHPLC HILIC column with 1.8 μm particle size, providing appropriate results for 22 out of the 24 species tested. In contrast, the detection of glyphosate and aminomethylphosphonic acid could only be achieved with a zwitterionic-type mixed-mode column, which proved to be suitable only for the pesticides detected in negative ion mode. Finally, the selected approach (UHPLC HILIC) was found to be useful for the determination of multiple pesticides in oranges using HILIC-ESI-MS/MS, with limits of quantitation in the low microgram per kilogram in most cases. [Figure not available: see fulltext.]

Original languageEnglish
Pages (from-to)1-13
Number of pages13
JournalAnalytical and Bioanalytical Chemistry
DOIs
Publication statusAccepted/In press - May 13 2016

Fingerprint

Liquid chromatography
Hydrophobic and Hydrophilic Interactions
Pesticides
Liquid Chromatography
Mass spectrometry
Mass Spectrometry
glyphosate
Silicon Dioxide
Particle Size
Ionization
Negative ions
Particle size
Ions

Keywords

  • Food
  • HILIC
  • LC-MS
  • Mixed mode
  • Pesticide testing
  • Polar pesticides

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry

Cite this

Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides. / Vass, Andrea; Robles-Molina, José; Pérez-Ortega, Patricia; Gilbert-López, Bienvenida; Dernovics, M.; Molina-Díaz, Antonio; García-Reyes, Juan F.

In: Analytical and Bioanalytical Chemistry, 13.05.2016, p. 1-13.

Research output: Contribution to journalArticle

Vass, Andrea ; Robles-Molina, José ; Pérez-Ortega, Patricia ; Gilbert-López, Bienvenida ; Dernovics, M. ; Molina-Díaz, Antonio ; García-Reyes, Juan F. / Study of different HILIC, mixed-mode, and other aqueous normal-phase approaches for the liquid chromatography/mass spectrometry-based determination of challenging polar pesticides. In: Analytical and Bioanalytical Chemistry. 2016 ; pp. 1-13.
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