Study of bovine herpesvirus type 1 strains with monoclonal antibodies.

L. Egyed, E. Brocchi, M. Rusvai, A. Bartha

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Fourteen strains of bovine herpesvirus type 1 (BHV-1, IBRV) representing all three groups of BHV-1 (BHV-1.1, BHV-1.2, BHV-1.3) were studied by ELISA using 106 monoclonal antibodies (Mabs) produced against BHV-1. On the basis of the ELISA, the Mabs could be divided into three groups. The first group (40 Mabs, 38%) reacted with all strains, the second group (43 Mabs, 41%) with the respiratory and genital strains (BHV-1.1 and BHV-1.2) while the third group (23 Mabs, 22%) only with the respiratory strains. Only 5 out of the antibodies neutralized respiratory and genital strains, and none of them neutralized the encephalitogenic strains (1.3). Three Mabs selected from each of the 3 groups, and the above five neutralizing strains were studied by Western blot. Antibodies of groups 1 and 3, and two neutralizing antibodies bound to a 90k protein (gpIII), whereas members of group 2 and 3 neutralizing antibodies reacted with a 74k and a 130k protein (both gpl). The results indicate that reactivity with monoclonal antibodies is as suitable for the classification of BHV-1 strains as is restriction endonuclease (RE) analysis but it cannot distinguish between subgroups within the groups.

Original languageEnglish
Pages (from-to)225-230
Number of pages6
JournalActa Veterinaria Hungarica
Volume40
Issue number3
Publication statusPublished - 1992

Fingerprint

Bovine Herpesvirus 1
monoclonal antibodies
Monoclonal Antibodies
cattle
Neutralizing Antibodies
neutralizing antibodies
genitalia
Enzyme-Linked Immunosorbent Assay
enzyme-linked immunosorbent assay
Antibodies
DNA Restriction Enzymes
antibodies
restriction mapping
neutralization
Proteins
Western Blotting
Western blotting
proteins

ASJC Scopus subject areas

  • veterinary(all)

Cite this

Study of bovine herpesvirus type 1 strains with monoclonal antibodies. / Egyed, L.; Brocchi, E.; Rusvai, M.; Bartha, A.

In: Acta Veterinaria Hungarica, Vol. 40, No. 3, 1992, p. 225-230.

Research output: Contribution to journalArticle

@article{0120f689cc07441bbf9a61bfd8836752,
title = "Study of bovine herpesvirus type 1 strains with monoclonal antibodies.",
abstract = "Fourteen strains of bovine herpesvirus type 1 (BHV-1, IBRV) representing all three groups of BHV-1 (BHV-1.1, BHV-1.2, BHV-1.3) were studied by ELISA using 106 monoclonal antibodies (Mabs) produced against BHV-1. On the basis of the ELISA, the Mabs could be divided into three groups. The first group (40 Mabs, 38{\%}) reacted with all strains, the second group (43 Mabs, 41{\%}) with the respiratory and genital strains (BHV-1.1 and BHV-1.2) while the third group (23 Mabs, 22{\%}) only with the respiratory strains. Only 5 out of the antibodies neutralized respiratory and genital strains, and none of them neutralized the encephalitogenic strains (1.3). Three Mabs selected from each of the 3 groups, and the above five neutralizing strains were studied by Western blot. Antibodies of groups 1 and 3, and two neutralizing antibodies bound to a 90k protein (gpIII), whereas members of group 2 and 3 neutralizing antibodies reacted with a 74k and a 130k protein (both gpl). The results indicate that reactivity with monoclonal antibodies is as suitable for the classification of BHV-1 strains as is restriction endonuclease (RE) analysis but it cannot distinguish between subgroups within the groups.",
author = "L. Egyed and E. Brocchi and M. Rusvai and A. Bartha",
year = "1992",
language = "English",
volume = "40",
pages = "225--230",
journal = "Acta Veterinaria Hungarica",
issn = "0236-6290",
publisher = "Akademiai Kiado",
number = "3",

}

TY - JOUR

T1 - Study of bovine herpesvirus type 1 strains with monoclonal antibodies.

AU - Egyed, L.

AU - Brocchi, E.

AU - Rusvai, M.

AU - Bartha, A.

PY - 1992

Y1 - 1992

N2 - Fourteen strains of bovine herpesvirus type 1 (BHV-1, IBRV) representing all three groups of BHV-1 (BHV-1.1, BHV-1.2, BHV-1.3) were studied by ELISA using 106 monoclonal antibodies (Mabs) produced against BHV-1. On the basis of the ELISA, the Mabs could be divided into three groups. The first group (40 Mabs, 38%) reacted with all strains, the second group (43 Mabs, 41%) with the respiratory and genital strains (BHV-1.1 and BHV-1.2) while the third group (23 Mabs, 22%) only with the respiratory strains. Only 5 out of the antibodies neutralized respiratory and genital strains, and none of them neutralized the encephalitogenic strains (1.3). Three Mabs selected from each of the 3 groups, and the above five neutralizing strains were studied by Western blot. Antibodies of groups 1 and 3, and two neutralizing antibodies bound to a 90k protein (gpIII), whereas members of group 2 and 3 neutralizing antibodies reacted with a 74k and a 130k protein (both gpl). The results indicate that reactivity with monoclonal antibodies is as suitable for the classification of BHV-1 strains as is restriction endonuclease (RE) analysis but it cannot distinguish between subgroups within the groups.

AB - Fourteen strains of bovine herpesvirus type 1 (BHV-1, IBRV) representing all three groups of BHV-1 (BHV-1.1, BHV-1.2, BHV-1.3) were studied by ELISA using 106 monoclonal antibodies (Mabs) produced against BHV-1. On the basis of the ELISA, the Mabs could be divided into three groups. The first group (40 Mabs, 38%) reacted with all strains, the second group (43 Mabs, 41%) with the respiratory and genital strains (BHV-1.1 and BHV-1.2) while the third group (23 Mabs, 22%) only with the respiratory strains. Only 5 out of the antibodies neutralized respiratory and genital strains, and none of them neutralized the encephalitogenic strains (1.3). Three Mabs selected from each of the 3 groups, and the above five neutralizing strains were studied by Western blot. Antibodies of groups 1 and 3, and two neutralizing antibodies bound to a 90k protein (gpIII), whereas members of group 2 and 3 neutralizing antibodies reacted with a 74k and a 130k protein (both gpl). The results indicate that reactivity with monoclonal antibodies is as suitable for the classification of BHV-1 strains as is restriction endonuclease (RE) analysis but it cannot distinguish between subgroups within the groups.

UR - http://www.scopus.com/inward/record.url?scp=0027014254&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027014254&partnerID=8YFLogxK

M3 - Article

C2 - 1284321

AN - SCOPUS:0027014254

VL - 40

SP - 225

EP - 230

JO - Acta Veterinaria Hungarica

JF - Acta Veterinaria Hungarica

SN - 0236-6290

IS - 3

ER -