Studies on the multiplicity of polypeptide hormones. I. Isolation of human pituitary growth hormone and characterization of the aggregates

L. Gráf, B. Szalontai, E. Barat, P. Závodszky, J. Borvendég, I. Hermann, G. Cseh

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3 Citations (Scopus)

Abstract

Growth hormone was prepared from acetone collected human pituitary glands by a simple new procedure. This preparation was resolved into 3 fractions by gel chromatography. The lowest molecular weight fraction was monomeric human growth hormone. The other 2 fractions contained different aggregated, biologically inactive forms of the hormone. Ultracentrifugal studies showed that these aggregates underwent dissociation in the presence of sodium dodecyl sulphate. The degree of dissociation was pH dependent.

Original languageEnglish
Pages (from-to)309-317
Number of pages9
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume9
Issue number4
Publication statusPublished - 1974

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Human Growth Hormone
Peptide Hormones
Pituitary Gland
Acetone
Sodium Dodecyl Sulfate
Growth Hormone
Gel Chromatography
Molecular Weight
Hormones

ASJC Scopus subject areas

  • Medicine(all)

Cite this

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abstract = "Growth hormone was prepared from acetone collected human pituitary glands by a simple new procedure. This preparation was resolved into 3 fractions by gel chromatography. The lowest molecular weight fraction was monomeric human growth hormone. The other 2 fractions contained different aggregated, biologically inactive forms of the hormone. Ultracentrifugal studies showed that these aggregates underwent dissociation in the presence of sodium dodecyl sulphate. The degree of dissociation was pH dependent.",
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AU - Gráf, L.

AU - Szalontai, B.

AU - Barat, E.

AU - Závodszky, P.

AU - Borvendég, J.

AU - Hermann, I.

AU - Cseh, G.

PY - 1974

Y1 - 1974

N2 - Growth hormone was prepared from acetone collected human pituitary glands by a simple new procedure. This preparation was resolved into 3 fractions by gel chromatography. The lowest molecular weight fraction was monomeric human growth hormone. The other 2 fractions contained different aggregated, biologically inactive forms of the hormone. Ultracentrifugal studies showed that these aggregates underwent dissociation in the presence of sodium dodecyl sulphate. The degree of dissociation was pH dependent.

AB - Growth hormone was prepared from acetone collected human pituitary glands by a simple new procedure. This preparation was resolved into 3 fractions by gel chromatography. The lowest molecular weight fraction was monomeric human growth hormone. The other 2 fractions contained different aggregated, biologically inactive forms of the hormone. Ultracentrifugal studies showed that these aggregates underwent dissociation in the presence of sodium dodecyl sulphate. The degree of dissociation was pH dependent.

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