Studies on molecular interactions between nalidixic acid and liposomes

M. Budai, Zs Szabó, A. Zimmer, M. Szögyi, P. Gróf

Research output: Contribution to journalArticle

24 Citations (Scopus)

Abstract

The interaction between nalidixic acid sodium salt (NANa) and liposomes prepared from α-L-dipalmitoyl-phosphatidylcholine (DPPC) or from its binary mixture with dioleoyl-phosphatidylcholine (DOPC) was studied with differential scanning calorimetry (DSC) and electron paramagnetic resonance (EPR) spectroscopy. We evaluated the role of broadband ultraviolet-B (UV-B) irradiation on the molecular interactions between the lipids and the NANa, and determined the decay-kinetics of the incorporated spin labeled fatty-acid free radicals. Multilamellar and unilamellar vesicles were prepared by sonication and extrusion. The entrapment efficiencies were determined spectrophotometrically. The size-distribution of the liposomes and its change in time was checked by dynamic light scattering (DLS). Our results indicate that NANa mainly interacts with lipid head groups. However, its effect and presumably the formation of the free radicals, induced by broadband ultraviolet-B, is not localized only to the head group region of the lipid molecules. Depending on DOPC content, interaction between the NANa and the lipids modifies the phase-transition parameters of the liposome dispersions.

Original languageEnglish
Pages (from-to)67-79
Number of pages13
JournalInternational Journal of Pharmaceutics
Volume279
Issue number1-2
DOIs
Publication statusPublished - Jul 26 2004

Fingerprint

Nalidixic Acid
Liposomes
Lipids
Free Radicals
1,2-Dipalmitoylphosphatidylcholine
Unilamellar Liposomes
Sonication
Phase Transition
Differential Scanning Calorimetry
Electron Spin Resonance Spectroscopy
Spectrum Analysis
Fatty Acids
Salts
Sodium
1,2-oleoylphosphatidylcholine

Keywords

  • DLS
  • DSC
  • EPR
  • Liposomes
  • Nalidixic acid
  • Zeta-potential

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

Studies on molecular interactions between nalidixic acid and liposomes. / Budai, M.; Szabó, Zs; Zimmer, A.; Szögyi, M.; Gróf, P.

In: International Journal of Pharmaceutics, Vol. 279, No. 1-2, 26.07.2004, p. 67-79.

Research output: Contribution to journalArticle

Budai, M. ; Szabó, Zs ; Zimmer, A. ; Szögyi, M. ; Gróf, P. / Studies on molecular interactions between nalidixic acid and liposomes. In: International Journal of Pharmaceutics. 2004 ; Vol. 279, No. 1-2. pp. 67-79.
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