A vérehulló fecskefucombining double acute accent (Chelidonium ma jus L.) tejedényeinek és tejnedvének vizsgálata fluoreszcencia képalkotási és fluoreszcencia spektroszkópiai módszerekkel

Translated title of the contribution: Studies on laticifers and milk of greater celandine (Chelidonium majus L.) with fluorescence imaging and fluorescence spectroscopic methods

Dorottya Póczi, B. Böddi

Research output: Contribution to journalArticle

Abstract

Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about the complexity of the milk and the various ratios of the alkaloid contents in the tissues. Whole plant were studied in a gel documentation system using ultraviolet light source, while the localisation of the laticifers was observed along the leaf veins in fluorescence microscope, using blue excitation light. Measuring different tissue pieces, fluorescence spectroscopic studies showed that the greater celandine alkaloids have emission bands at 469, 530-531, 553, 572-575 and 592 nm and excitation bands at 365, 370, 386 és 400 nm. These results give a possibility for conclusions about the alkaloid contents and composition or ratios of the alkaloid components in various tissue pieces directly, via comparisons with alkaloid standards.

Original languageHungarian
Pages (from-to)95-100
Number of pages6
JournalActa Pharmaceutica Hungarica
Volume80
Issue number3
Publication statusPublished - 2010

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Chelidonium
Optical Imaging
Alkaloids
Milk
Fluorescence
Ultraviolet Rays
Documentation
Veins
Gels
Light

ASJC Scopus subject areas

  • Pharmaceutical Science

Cite this

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title = "A v{\'e}rehull{\'o} fecskefucombining double acute accent (Chelidonium ma jus L.) tejed{\'e}nyeinek {\'e}s tejnedv{\'e}nek vizsg{\'a}lata fluoreszcencia k{\'e}palkot{\'a}si {\'e}s fluoreszcencia spektroszk{\'o}piai m{\'o}dszerekkel",
abstract = "Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about the complexity of the milk and the various ratios of the alkaloid contents in the tissues. Whole plant were studied in a gel documentation system using ultraviolet light source, while the localisation of the laticifers was observed along the leaf veins in fluorescence microscope, using blue excitation light. Measuring different tissue pieces, fluorescence spectroscopic studies showed that the greater celandine alkaloids have emission bands at 469, 530-531, 553, 572-575 and 592 nm and excitation bands at 365, 370, 386 {\'e}s 400 nm. These results give a possibility for conclusions about the alkaloid contents and composition or ratios of the alkaloid components in various tissue pieces directly, via comparisons with alkaloid standards.",
keywords = "Chelidonium majus L, Fluorescence imaging, Fluorescence spectroscopy, Greater celandine, Milk-alkaloids",
author = "Dorottya P{\'o}czi and B. B{\"o}ddi",
year = "2010",
language = "Hungarian",
volume = "80",
pages = "95--100",
journal = "Acta Pharmaceutica Hungarica",
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publisher = "Magyar Gyogyszereszeti Tarsasag",
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TY - JOUR

T1 - A vérehulló fecskefucombining double acute accent (Chelidonium ma jus L.) tejedényeinek és tejnedvének vizsgálata fluoreszcencia képalkotási és fluoreszcencia spektroszkópiai módszerekkel

AU - Póczi, Dorottya

AU - Böddi, B.

PY - 2010

Y1 - 2010

N2 - Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about the complexity of the milk and the various ratios of the alkaloid contents in the tissues. Whole plant were studied in a gel documentation system using ultraviolet light source, while the localisation of the laticifers was observed along the leaf veins in fluorescence microscope, using blue excitation light. Measuring different tissue pieces, fluorescence spectroscopic studies showed that the greater celandine alkaloids have emission bands at 469, 530-531, 553, 572-575 and 592 nm and excitation bands at 365, 370, 386 és 400 nm. These results give a possibility for conclusions about the alkaloid contents and composition or ratios of the alkaloid components in various tissue pieces directly, via comparisons with alkaloid standards.

AB - Using fluorescence imaging and fluorescence spectroscopic methods, the localisation of the laticifers and the native spectral properties of the milk were studied in various organs of greater celandine (Chelidonium majus L.). Direct measurements on tissue pieces (without the extraction and the separation of the components) provided information about the complexity of the milk and the various ratios of the alkaloid contents in the tissues. Whole plant were studied in a gel documentation system using ultraviolet light source, while the localisation of the laticifers was observed along the leaf veins in fluorescence microscope, using blue excitation light. Measuring different tissue pieces, fluorescence spectroscopic studies showed that the greater celandine alkaloids have emission bands at 469, 530-531, 553, 572-575 and 592 nm and excitation bands at 365, 370, 386 és 400 nm. These results give a possibility for conclusions about the alkaloid contents and composition or ratios of the alkaloid components in various tissue pieces directly, via comparisons with alkaloid standards.

KW - Chelidonium majus L

KW - Fluorescence imaging

KW - Fluorescence spectroscopy

KW - Greater celandine

KW - Milk-alkaloids

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