Studies on human tonsillar lymphocyte membrane III. Isolation and characterization of plasma membrane fractions after hypotonic lysis

A. Hrabak, M. T. Szabo, F. Antoni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume16
Issue number1-2
Publication statusPublished - Dec 1 1981

ASJC Scopus subject areas

  • Medicine(all)

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