Studies on human tonsillar lymphocyte membrane III. Isolation and characterization of plasma membrane fractions after hypotonic lysis

A. Hrabák, M. T. Szabo, F. Antoni

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.

Original languageEnglish
Pages (from-to)41-49
Number of pages9
JournalActa Biochimica et Biophysica Academiae Scientiarum Hungaricae
Volume16
Issue number1-2
Publication statusPublished - 1981

Fingerprint

Cell Membrane
Lymphocytes
Reticulum
Membranes
N-Acetylneuraminic Acid
Phospholipids
Cholesterol
DNA
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

@article{0214c581d8bf435d848290a34536be42,
title = "Studies on human tonsillar lymphocyte membrane III. Isolation and characterization of plasma membrane fractions after hypotonic lysis",
abstract = "Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.",
author = "A. Hrab{\'a}k and Szabo, {M. T.} and F. Antoni",
year = "1981",
language = "English",
volume = "16",
pages = "41--49",
journal = "Ideggyogyaszati Szemle",
issn = "0019-1442",
publisher = "Ifjusagi Lap-es Konyvkiado Vallalat",
number = "1-2",

}

TY - JOUR

T1 - Studies on human tonsillar lymphocyte membrane III. Isolation and characterization of plasma membrane fractions after hypotonic lysis

AU - Hrabák, A.

AU - Szabo, M. T.

AU - Antoni, F.

PY - 1981

Y1 - 1981

N2 - Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.

AB - Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.

UR - http://www.scopus.com/inward/record.url?scp=0019855065&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0019855065&partnerID=8YFLogxK

M3 - Article

C2 - 7336883

AN - SCOPUS:0019855065

VL - 16

SP - 41

EP - 49

JO - Ideggyogyaszati Szemle

JF - Ideggyogyaszati Szemle

SN - 0019-1442

IS - 1-2

ER -