Hypotonic lysis was applied for the preparation of lymphocyte plasma membranes. By this method it was possible to separate membrane fractions with a high sialic acid to protein and cholesterol to phospholipid ratio. The plasma membrane fractions had a lower content of endoplasmatic reticulum than the preparations described earlier but were slightly contaminated with DNA. The isolation method proved to be useful for the preparation of surface membranes from human lymphocytes.
|Number of pages||9|
|Journal||Acta Biochimica et Biophysica Academiae Scientiarum Hungaricae|
|Publication status||Published - Dec 1 1981|
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