Studies on Feeding and Digestion in Protozoa. VII. Ingestion of Polystyrene Latex Particles and its Early Effect on Acid Phosphatase in Paramecium multimicronucleatum and Tetrahymena pyriformis

M. MUELLER, P. Röhlich, I. TÖRÖ

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40 Citations (Scopus)

Abstract

SYNOPSIS. Food vacuole‐free P. multimicronucleatum and T. pyriformis readily ingest non‐nutritive Dow polystyrene latex particles (PLP) and form vacuoles containing PLP at a rate comparable to the formation of vacuoles containing bacteria. The particles aggregate within the vacuoles and are egested as balls of the size of the vacuoles. PLP containing vacuoles rapidly acquire acid phosphatase activity, which is demonstrated by histochemical (alpha‐naphthyl phosphatehexazonium salt or lead phosphate) methods as a peripheric staining. The total activity of the cell does not significantly change as a consequence of PLP uptake as suggested by the histochemical preparations and confirmed in T. pyriformis by measuring the splitting of p‐nitrophenyl phosphate at pH 5. Accordingly, no selection between nutritive and non‐nutritive particles could be revealed. The vacuole formation is induced by the mechanical action of the particles. The appearance of acid phosphatase activity in the vacuole seems to be dependent on the vacuole formation and not on its content. This early appearance of activity is due to a redistribution of the preexistent activity.

Original languageEnglish
Pages (from-to)27-34
Number of pages8
JournalThe Journal of Protozoology
Volume12
Issue number1
DOIs
Publication statusPublished - 1965

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Paramecium
Tetrahymena pyriformis
Acid Phosphatase
Vacuoles
Microspheres
Digestion
Eating
styrofoam
Salts
Phosphates
Staining and Labeling
Bacteria
Food

ASJC Scopus subject areas

  • Parasitology

Cite this

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title = "Studies on Feeding and Digestion in Protozoa. VII. Ingestion of Polystyrene Latex Particles and its Early Effect on Acid Phosphatase in Paramecium multimicronucleatum and Tetrahymena pyriformis",
abstract = "SYNOPSIS. Food vacuole‐free P. multimicronucleatum and T. pyriformis readily ingest non‐nutritive Dow polystyrene latex particles (PLP) and form vacuoles containing PLP at a rate comparable to the formation of vacuoles containing bacteria. The particles aggregate within the vacuoles and are egested as balls of the size of the vacuoles. PLP containing vacuoles rapidly acquire acid phosphatase activity, which is demonstrated by histochemical (alpha‐naphthyl phosphatehexazonium salt or lead phosphate) methods as a peripheric staining. The total activity of the cell does not significantly change as a consequence of PLP uptake as suggested by the histochemical preparations and confirmed in T. pyriformis by measuring the splitting of p‐nitrophenyl phosphate at pH 5. Accordingly, no selection between nutritive and non‐nutritive particles could be revealed. The vacuole formation is induced by the mechanical action of the particles. The appearance of acid phosphatase activity in the vacuole seems to be dependent on the vacuole formation and not on its content. This early appearance of activity is due to a redistribution of the preexistent activity.",
author = "M. MUELLER and P. R{\"o}hlich and I. T{\"O}R{\"O}",
year = "1965",
doi = "10.1111/j.1550-7408.1965.tb01807.x",
language = "English",
volume = "12",
pages = "27--34",
journal = "Journal of Eukaryotic Microbiology",
issn = "1066-5234",
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T1 - Studies on Feeding and Digestion in Protozoa. VII. Ingestion of Polystyrene Latex Particles and its Early Effect on Acid Phosphatase in Paramecium multimicronucleatum and Tetrahymena pyriformis

AU - MUELLER, M.

AU - Röhlich, P.

AU - TÖRÖ, I.

PY - 1965

Y1 - 1965

N2 - SYNOPSIS. Food vacuole‐free P. multimicronucleatum and T. pyriformis readily ingest non‐nutritive Dow polystyrene latex particles (PLP) and form vacuoles containing PLP at a rate comparable to the formation of vacuoles containing bacteria. The particles aggregate within the vacuoles and are egested as balls of the size of the vacuoles. PLP containing vacuoles rapidly acquire acid phosphatase activity, which is demonstrated by histochemical (alpha‐naphthyl phosphatehexazonium salt or lead phosphate) methods as a peripheric staining. The total activity of the cell does not significantly change as a consequence of PLP uptake as suggested by the histochemical preparations and confirmed in T. pyriformis by measuring the splitting of p‐nitrophenyl phosphate at pH 5. Accordingly, no selection between nutritive and non‐nutritive particles could be revealed. The vacuole formation is induced by the mechanical action of the particles. The appearance of acid phosphatase activity in the vacuole seems to be dependent on the vacuole formation and not on its content. This early appearance of activity is due to a redistribution of the preexistent activity.

AB - SYNOPSIS. Food vacuole‐free P. multimicronucleatum and T. pyriformis readily ingest non‐nutritive Dow polystyrene latex particles (PLP) and form vacuoles containing PLP at a rate comparable to the formation of vacuoles containing bacteria. The particles aggregate within the vacuoles and are egested as balls of the size of the vacuoles. PLP containing vacuoles rapidly acquire acid phosphatase activity, which is demonstrated by histochemical (alpha‐naphthyl phosphatehexazonium salt or lead phosphate) methods as a peripheric staining. The total activity of the cell does not significantly change as a consequence of PLP uptake as suggested by the histochemical preparations and confirmed in T. pyriformis by measuring the splitting of p‐nitrophenyl phosphate at pH 5. Accordingly, no selection between nutritive and non‐nutritive particles could be revealed. The vacuole formation is induced by the mechanical action of the particles. The appearance of acid phosphatase activity in the vacuole seems to be dependent on the vacuole formation and not on its content. This early appearance of activity is due to a redistribution of the preexistent activity.

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