Studies on elemental composition and antioxidant capacity in callus cultures and native plants of Vaccinium myrtillus L. Local populations

Vanda Veres Bolda, Dorica Botau, Réka Szollosi, Andrea Peto, Ágnes Gallé, I. Tari

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

The biological and medical effects of bilberry fruit (Vaccinium myrtillus L.) are mainly due to high anthocyanin content of tissues. Calli containing anthocyanins, derived from bilberry plants, may represent a potential source of natural colouring matter, pharmaceutical and natural antioxidants. In the present study we investigated the occurrence of differences in elemental composition and antioxidant capacity of the three local populations of mountain bilberry collected in the western region of Romania (Arieseni, Retezat and Sebes Valley) in order to compare the anthocyanin production of plant and callus tissues originated from various plant populations. It was found that K, Fe and Zn content was higher in calli than in intact plant leaves. The excess of latter two microelements, Fe and Zn can induce oxidative stress, and, as a result of the accumulation of reactive oxygen species, various antioxidant mechanisms. The total antioxidant capacity of callus cultures determined by FRAP method (ferric reducing antioxidant power) could be enhanced as a function of increasing adenine sulphate (AS) concentration in the culture medium and it depended on the origin of mother plants. The leaves of intact plants contained higher amount of total non-protein sulfhydryl groups than calli, and the decrease was especially significant in tissue cultures originated from the Retezat region. In contrast, depending on the AS concentration, the anthocyanin content could increase in callus cultures. The tissues originated from various populations exhibited different AS concentration optimum. This suggests that bilberry callus cultures can be a suitable source of the anthocyanins.

Original languageEnglish
Pages (from-to)255-259
Number of pages5
JournalActa Biologica Szegediensis
Volume55
Issue number2
Publication statusPublished - 2011

Fingerprint

Vaccinium myrtillus
Anthocyanins
Bony Callus
callus culture
bilberries
Antioxidants
anthocyanins
antioxidants
Adenine
Sulfates
callus
adenine
Chemical analysis
Population
Tissue
sulfates
Plant Leaves
Tissue culture
Oxidative stress
Coloring

Keywords

  • Anthocyanins
  • Antioxidant capacity
  • Atomic absorption
  • Callus cultures
  • Intact plants
  • Spectrometry
  • Vaccinium myrtillus L

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Studies on elemental composition and antioxidant capacity in callus cultures and native plants of Vaccinium myrtillus L. Local populations. / Bolda, Vanda Veres; Botau, Dorica; Szollosi, Réka; Peto, Andrea; Gallé, Ágnes; Tari, I.

In: Acta Biologica Szegediensis, Vol. 55, No. 2, 2011, p. 255-259.

Research output: Contribution to journalArticle

Bolda, Vanda Veres ; Botau, Dorica ; Szollosi, Réka ; Peto, Andrea ; Gallé, Ágnes ; Tari, I. / Studies on elemental composition and antioxidant capacity in callus cultures and native plants of Vaccinium myrtillus L. Local populations. In: Acta Biologica Szegediensis. 2011 ; Vol. 55, No. 2. pp. 255-259.
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