Studies on acute myelomonocytic leukemia in LBF1 rats

L. Kozma, A. Kiss, I. Ember, P. Kertai

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Granulocytic leukemia was induced in Long-Evans (LE) rats by using the Huggins and Sugiyama method. After serial passage the cells became transformed. The newly transformed cells could be transplanted to LBF1 hybrid rats and observed more readily. A quantity of 108 cells/100 g body weight was injected intravenously and after 2 - 3 weeks myelomonocytic leukemia developed. By examining the bone marrow, spleen and lymph nodes, cytochemical tests verified this transformation. Transplanting 102 - 104 cells under the renal capsule, a quickly growing solid tumor was observed, which caused metastasis to the parathymical lymph nodes and peritoneum. The investigation of oncogene expression for the myc and ras families revealed the presence of myc p62 and ras p21 oncoproteins in the tumor cells by using monoclonal antibodies in immunohistochemical tests. LBF1 rats proved to be good models in obtaining solid tumor growth and myelomonocytic leukemias, equivalent to human M4 - M5 type leukemia.

Original languageEnglish
Pages (from-to)185-192
Number of pages8
JournalCancer Letters
Volume68
Issue number2-3
DOIs
Publication statusPublished - 1993

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Leukemia, Myelomonocytic, Acute
Leukemia
Lymph Nodes
Proto-Oncogene Proteins p21(ras)
Serial Passage
Neoplasms
Long Evans Rats
myc Genes
Myeloid Leukemia
ras Genes
Peritoneum
Oncogene Proteins
Capsules
Spleen
Bone Marrow
Monoclonal Antibodies
Body Weight
Neoplasm Metastasis
Kidney
Growth

Keywords

  • experimental myelomonocytic leukemia
  • experimental myelomonocytoma

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Studies on acute myelomonocytic leukemia in LBF1 rats. / Kozma, L.; Kiss, A.; Ember, I.; Kertai, P.

In: Cancer Letters, Vol. 68, No. 2-3, 1993, p. 185-192.

Research output: Contribution to journalArticle

Kozma, L. ; Kiss, A. ; Ember, I. ; Kertai, P. / Studies on acute myelomonocytic leukemia in LBF1 rats. In: Cancer Letters. 1993 ; Vol. 68, No. 2-3. pp. 185-192.
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