Studies on a possible molecular basis for the structure of mitochondrial cristae.

B. Sümegi, D. A. Freeman, L. Inman, P. Srere

Research output: Contribution to journalArticle

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Abstract

We have investigated a possible molecular basis for mitochondrial cristae formation. Proteoliposomes containing electron transport proteins, cytochrome oxidase, or complex III in their proper orientation bind to pig heart mitoplasts but not pig heart mitochondria. Using Leydig tumor cells, we have confirmed earlier reports that chloramphenicol causes a diminution in cristae content and a change in its characteristic lamellar form. We show that the proteoliposomes containing cytochrome oxidase or complex III in the proper orientation bind to mitoplasts from Leydig tumor cells but do not bind as well to mitoplasts from chloramphenicol-treated Leydig tumor cells. These experiments provide a possible mechanism to explain cristae formation.

Original languageEnglish
Pages (from-to)19-24
Number of pages6
JournalJournal of Molecular Recognition
Volume1
Issue number1
Publication statusPublished - Feb 1988

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Leydig Cell Tumor
Molecular Structure
Tumors
Electron Transport Complex III
Cells
Electron Transport Complex IV
Chloramphenicol
Swine
Heart Mitochondria
Mitochondria
Electron Transport
Carrier Proteins
Experiments
proteoliposomes

ASJC Scopus subject areas

  • Biochemistry

Cite this

Studies on a possible molecular basis for the structure of mitochondrial cristae. / Sümegi, B.; Freeman, D. A.; Inman, L.; Srere, P.

In: Journal of Molecular Recognition, Vol. 1, No. 1, 02.1988, p. 19-24.

Research output: Contribution to journalArticle

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