Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR

György Pósfai, Frank Baldauf, Sára Erdei, Jánós Pósfai, Pál Venetianer, Antal Kiss

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The nucleotide sequence of the gene coding for the 5′-GGCC and 5′-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5′-GGCC-specific BspRI modification methylase.

Original languageEnglish
Pages (from-to)9039-9049
Number of pages11
JournalNucleic acids research
Volume12
Issue number23
DOIs
Publication statusPublished - Dec 11 1984

ASJC Scopus subject areas

  • Genetics

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