Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR

György Pósfai, Frank Baldauf, Sára Erdei, Jánós Pósfai, Pál Venetianer, Antal Kiss

Research output: Contribution to journalArticle

42 Citations (Scopus)

Abstract

The nucleotide sequence of the gene coding for the 5′-GGCC and 5′-CCGG specific DNA methyltransferase of the Bacillus subtilis phage SPR was determined by the Maxam-Gilbert procedure. Transcriptional and translational signals of the sequence were assigned with the help of S1 mapping and translation in E. coli minicells. The gene codes for a 49 kd polypeptide. The amino acid sequence of the SPR methylase shows regions of homology with the sequence of the 5′-GGCC-specific BspRI modification methylase.

Original languageEnglish
Pages (from-to)9039-9049
Number of pages11
JournalNucleic Acids Research
Volume12
Issue number23
DOIs
Publication statusPublished - Dec 11 1984

Fingerprint

Bacteriophages
Methyltransferases
DNA Modification Methylases
DNA
Coding
Genes
Bacillus Phages
Gene
Polypeptides
Bacilli
Sequence Homology
Nucleotides
Protein Sorting Signals
Bacillus subtilis
Escherichia coli
Amino acids
Amino Acid Sequence
Amino Acids
Peptides
Escherichia Coli

ASJC Scopus subject areas

  • Statistics, Probability and Uncertainty
  • Applied Mathematics
  • Health, Toxicology and Mutagenesis
  • Toxicology
  • Genetics(clinical)
  • Genetics

Cite this

Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR. / Pósfai, György; Baldauf, Frank; Erdei, Sára; Pósfai, Jánós; Venetianer, Pál; Kiss, Antal.

In: Nucleic Acids Research, Vol. 12, No. 23, 11.12.1984, p. 9039-9049.

Research output: Contribution to journalArticle

Pósfai, György ; Baldauf, Frank ; Erdei, Sára ; Pósfai, Jánós ; Venetianer, Pál ; Kiss, Antal. / Structure of the gene coding for the sequence-specific DNA-methyltransferase of the B. subtilis phage SPR. In: Nucleic Acids Research. 1984 ; Vol. 12, No. 23. pp. 9039-9049.
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