Structure and enzymatic mechanism of a moonlighting dUTPase

Ibolya Leveles, Veronika Németh, Judit E. Szabó, V. Harmat, Kinga Nyíri, Ábris Ádám Bendes, Veronika Papp-Kádár, Imre Zagyva, Gergely Róna, Olivér Ozohanics, K. Vékey, J. Tóth, B. Vértessy

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Genome integrity requires well controlled cellular pools of nucleotides. dUTPases are responsible for regulating cellular dUTP levels and providing dUMP for dTTP biosynthesis. In Staphylococcus, phage dUTPases are also suggested to be involved in a moonlighting function regulating the expression of pathogenicity-island genes. Staphylococcal phage trimeric dUTPase sequences include a specific insertion that is not found in other organisms. Here, a 2.1 Å resolution three-dimensional structure of a φ11 phage dUTPase trimer with complete localization of the phage-specific insert, which folds into a small β-pleated mini-domain reaching out from the dUTPase core surface, is presented. The insert mini-domains jointly coordinate a single Mg2+ ion per trimer at the entrance to the threefold inner channel. Structural results provide an explanation for the role of Asp95, which is suggested to have functional significance in the moonlighting activity, as the metal-ion-coordinating moiety potentially involved in correct positioning of the insert. Enzyme-kinetics studies of wild-type and mutant constructs show that the insert has no major role in dUTP binding or cleavage and provide a description of the elementary steps (fast binding of substrate and release of products). In conclusion, the structural and kinetic data allow insights into both the phage-specific characteristics and the generally conserved traits of φ11 phage dUTPase.

Original languageEnglish
Pages (from-to)2298-2308
Number of pages11
JournalActa Crystallographica Section D: Biological Crystallography
Volume69
Issue number12
DOIs
Publication statusPublished - Dec 2013

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Bacteriophages
Staphylococcus Phages
Ions
Genomic Islands
Staphylococcus
Nucleotides
Metals
dUTP pyrophosphatase
Genome
Enzymes
Genes

Keywords

  • φ11 phage dUTPase
  • dUTPases
  • moonlighting

ASJC Scopus subject areas

  • Structural Biology

Cite this

Structure and enzymatic mechanism of a moonlighting dUTPase. / Leveles, Ibolya; Németh, Veronika; Szabó, Judit E.; Harmat, V.; Nyíri, Kinga; Bendes, Ábris Ádám; Papp-Kádár, Veronika; Zagyva, Imre; Róna, Gergely; Ozohanics, Olivér; Vékey, K.; Tóth, J.; Vértessy, B.

In: Acta Crystallographica Section D: Biological Crystallography, Vol. 69, No. 12, 12.2013, p. 2298-2308.

Research output: Contribution to journalArticle

Leveles, I, Németh, V, Szabó, JE, Harmat, V, Nyíri, K, Bendes, ÁÁ, Papp-Kádár, V, Zagyva, I, Róna, G, Ozohanics, O, Vékey, K, Tóth, J & Vértessy, B 2013, 'Structure and enzymatic mechanism of a moonlighting dUTPase', Acta Crystallographica Section D: Biological Crystallography, vol. 69, no. 12, pp. 2298-2308. https://doi.org/10.1107/S0907444913021136
Leveles, Ibolya ; Németh, Veronika ; Szabó, Judit E. ; Harmat, V. ; Nyíri, Kinga ; Bendes, Ábris Ádám ; Papp-Kádár, Veronika ; Zagyva, Imre ; Róna, Gergely ; Ozohanics, Olivér ; Vékey, K. ; Tóth, J. ; Vértessy, B. / Structure and enzymatic mechanism of a moonlighting dUTPase. In: Acta Crystallographica Section D: Biological Crystallography. 2013 ; Vol. 69, No. 12. pp. 2298-2308.
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