Structure and biological activity of pathogen-like synthetic nanomedicines

Orsolya Lorincz, Eniko R. Toke, Eszter Somogyi, Ferenc Horkay, Preethi L. Chandran, Jack F. Douglas, J. Szebeni, Julianna Lisziewicz

Research output: Contribution to journalArticle

21 Citations (Scopus)

Abstract

Here we characterize the structure, stability and intracellular mode of action of DermaVir nanomedicine that is under clinical development for the treatment of HIV/AIDS. This nanomedicine comprises pathogen-like pDNA/PEIm nanoparticles (NPs) having the structure and function resembling spherical viruses that naturally evolved to deliver nucleic acids to the cells. Atomic force microscopy demonstrated spherical 100 - 200 nm NPs with a smooth polymer surface protecting the pDNA in the core. Optical absorption determined both the NP structural stability and biological activity relevant to their ability to escape from the endosome and release the pDNA at the nucleus. Salt, pH and temperature influence nanomedicine shelf-life and intracellular stability. This approach facilitates the development of diverse polyplex nanomedicines where the delivered pDNA-expressed antigens induce immune responses to kill infected cells. From the Clinical Editor: The authors investigated DermaVir nanomedicine comprised of pathogen-like pDNA/PEIm nanoparticles with structure and function resembling spherical viruses. DermaVir delivery of pDNA expresses antigens that induce immune responses to kill HIV infected cells.

Original languageEnglish
Pages (from-to)497-506
Number of pages10
JournalNanomedicine: Nanotechnology, Biology, and Medicine
Volume8
Issue number4
DOIs
Publication statusPublished - May 2012

Fingerprint

Nanomedicine
Medical nanotechnology
Pathogens
Bioactivity
Nanoparticles
Histocompatibility Antigens Class II
Antigens
Viruses
HIV
Atomic Force Microscopy
Endosomes
Nucleic acids
Light absorption
Nucleic Acids
Atomic force microscopy
Polymers
Acquired Immunodeficiency Syndrome
Salts
Temperature
DermaVir

Keywords

  • DermaVir
  • Immunity
  • Immunotherapy
  • Vaccine

ASJC Scopus subject areas

  • Molecular Medicine
  • Bioengineering
  • Biomedical Engineering
  • Materials Science(all)
  • Medicine (miscellaneous)
  • Pharmaceutical Science

Cite this

Lorincz, O., Toke, E. R., Somogyi, E., Horkay, F., Chandran, P. L., Douglas, J. F., ... Lisziewicz, J. (2012). Structure and biological activity of pathogen-like synthetic nanomedicines. Nanomedicine: Nanotechnology, Biology, and Medicine, 8(4), 497-506. https://doi.org/10.1016/j.nano.2011.07.013

Structure and biological activity of pathogen-like synthetic nanomedicines. / Lorincz, Orsolya; Toke, Eniko R.; Somogyi, Eszter; Horkay, Ferenc; Chandran, Preethi L.; Douglas, Jack F.; Szebeni, J.; Lisziewicz, Julianna.

In: Nanomedicine: Nanotechnology, Biology, and Medicine, Vol. 8, No. 4, 05.2012, p. 497-506.

Research output: Contribution to journalArticle

Lorincz, O, Toke, ER, Somogyi, E, Horkay, F, Chandran, PL, Douglas, JF, Szebeni, J & Lisziewicz, J 2012, 'Structure and biological activity of pathogen-like synthetic nanomedicines', Nanomedicine: Nanotechnology, Biology, and Medicine, vol. 8, no. 4, pp. 497-506. https://doi.org/10.1016/j.nano.2011.07.013
Lorincz, Orsolya ; Toke, Eniko R. ; Somogyi, Eszter ; Horkay, Ferenc ; Chandran, Preethi L. ; Douglas, Jack F. ; Szebeni, J. ; Lisziewicz, Julianna. / Structure and biological activity of pathogen-like synthetic nanomedicines. In: Nanomedicine: Nanotechnology, Biology, and Medicine. 2012 ; Vol. 8, No. 4. pp. 497-506.
@article{4c179977e1854e3182a13f3f1f35a6db,
title = "Structure and biological activity of pathogen-like synthetic nanomedicines",
abstract = "Here we characterize the structure, stability and intracellular mode of action of DermaVir nanomedicine that is under clinical development for the treatment of HIV/AIDS. This nanomedicine comprises pathogen-like pDNA/PEIm nanoparticles (NPs) having the structure and function resembling spherical viruses that naturally evolved to deliver nucleic acids to the cells. Atomic force microscopy demonstrated spherical 100 - 200 nm NPs with a smooth polymer surface protecting the pDNA in the core. Optical absorption determined both the NP structural stability and biological activity relevant to their ability to escape from the endosome and release the pDNA at the nucleus. Salt, pH and temperature influence nanomedicine shelf-life and intracellular stability. This approach facilitates the development of diverse polyplex nanomedicines where the delivered pDNA-expressed antigens induce immune responses to kill infected cells. From the Clinical Editor: The authors investigated DermaVir nanomedicine comprised of pathogen-like pDNA/PEIm nanoparticles with structure and function resembling spherical viruses. DermaVir delivery of pDNA expresses antigens that induce immune responses to kill HIV infected cells.",
keywords = "DermaVir, Immunity, Immunotherapy, Vaccine",
author = "Orsolya Lorincz and Toke, {Eniko R.} and Eszter Somogyi and Ferenc Horkay and Chandran, {Preethi L.} and Douglas, {Jack F.} and J. Szebeni and Julianna Lisziewicz",
year = "2012",
month = "5",
doi = "10.1016/j.nano.2011.07.013",
language = "English",
volume = "8",
pages = "497--506",
journal = "Nanomedicine: Nanotechnology, Biology, and Medicine",
issn = "1549-9634",
publisher = "Elsevier Inc.",
number = "4",

}

TY - JOUR

T1 - Structure and biological activity of pathogen-like synthetic nanomedicines

AU - Lorincz, Orsolya

AU - Toke, Eniko R.

AU - Somogyi, Eszter

AU - Horkay, Ferenc

AU - Chandran, Preethi L.

AU - Douglas, Jack F.

AU - Szebeni, J.

AU - Lisziewicz, Julianna

PY - 2012/5

Y1 - 2012/5

N2 - Here we characterize the structure, stability and intracellular mode of action of DermaVir nanomedicine that is under clinical development for the treatment of HIV/AIDS. This nanomedicine comprises pathogen-like pDNA/PEIm nanoparticles (NPs) having the structure and function resembling spherical viruses that naturally evolved to deliver nucleic acids to the cells. Atomic force microscopy demonstrated spherical 100 - 200 nm NPs with a smooth polymer surface protecting the pDNA in the core. Optical absorption determined both the NP structural stability and biological activity relevant to their ability to escape from the endosome and release the pDNA at the nucleus. Salt, pH and temperature influence nanomedicine shelf-life and intracellular stability. This approach facilitates the development of diverse polyplex nanomedicines where the delivered pDNA-expressed antigens induce immune responses to kill infected cells. From the Clinical Editor: The authors investigated DermaVir nanomedicine comprised of pathogen-like pDNA/PEIm nanoparticles with structure and function resembling spherical viruses. DermaVir delivery of pDNA expresses antigens that induce immune responses to kill HIV infected cells.

AB - Here we characterize the structure, stability and intracellular mode of action of DermaVir nanomedicine that is under clinical development for the treatment of HIV/AIDS. This nanomedicine comprises pathogen-like pDNA/PEIm nanoparticles (NPs) having the structure and function resembling spherical viruses that naturally evolved to deliver nucleic acids to the cells. Atomic force microscopy demonstrated spherical 100 - 200 nm NPs with a smooth polymer surface protecting the pDNA in the core. Optical absorption determined both the NP structural stability and biological activity relevant to their ability to escape from the endosome and release the pDNA at the nucleus. Salt, pH and temperature influence nanomedicine shelf-life and intracellular stability. This approach facilitates the development of diverse polyplex nanomedicines where the delivered pDNA-expressed antigens induce immune responses to kill infected cells. From the Clinical Editor: The authors investigated DermaVir nanomedicine comprised of pathogen-like pDNA/PEIm nanoparticles with structure and function resembling spherical viruses. DermaVir delivery of pDNA expresses antigens that induce immune responses to kill HIV infected cells.

KW - DermaVir

KW - Immunity

KW - Immunotherapy

KW - Vaccine

UR - http://www.scopus.com/inward/record.url?scp=84860238423&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84860238423&partnerID=8YFLogxK

U2 - 10.1016/j.nano.2011.07.013

DO - 10.1016/j.nano.2011.07.013

M3 - Article

C2 - 21839051

AN - SCOPUS:84860238423

VL - 8

SP - 497

EP - 506

JO - Nanomedicine: Nanotechnology, Biology, and Medicine

JF - Nanomedicine: Nanotechnology, Biology, and Medicine

SN - 1549-9634

IS - 4

ER -