Structure analysis of proteins, peptides and metal complexes by vibrational circular dichroism

M. Hollósi, E. Vass, Gábor Szilvágyi, Andreja Jakas, I. Laczkó

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

There are two principal forms of vibrational optical activity (VOA), an IR form referred to as vibrational circular dichroism (VCD) and Raman form known as Raman optical activity (ROA). This paper reports examples of the application of VCD spectroscopy for the determination of the absolute configuration and conformation of chiral molecules, e.g. cyclic β-lactams. VCD spectroscopy can be applied for the characterization of the conformation of proteins and peptides in solution. VCD based conformational analysis of cyclic peptides is discussed. Examples are the cyclic hexapeptide cyclo ( Pro2-Gly- Pro2-Gly) and cyclic peptides comprising β-omoamino acids (trans -2-aminocyclopentane or trans-2-aminocyclohexane carboxylic acid). Structure analysis by VCD of opiate peptides, glycopeptides, peptidomimetics and chiral transition metal complexes are also discussed.

Original languageEnglish
Pages (from-to)291-300
Number of pages10
JournalArkivoc
Volume2012
Issue number5
DOIs
Publication statusPublished - Jun 8 2012

Fingerprint

Coordination Complexes
Peptides
Circular dichroism spectroscopy
Vibrational spectroscopy
Cyclic Peptides
Proteins
Conformations
Peptidomimetics
Lactams
Opioid Peptides
Glycopeptides
Carboxylic Acids
Transition metals
Circular Dichroism
Molecules
Acids

Keywords

  • Characterization of protein conformation
  • Determination of absolute configuration
  • Structure analysis of cyclic peptides
  • VCD of glycopeptides and chiral transition metal complexes
  • Vibrational circular dichroism (VCD)

ASJC Scopus subject areas

  • Organic Chemistry

Cite this

Structure analysis of proteins, peptides and metal complexes by vibrational circular dichroism. / Hollósi, M.; Vass, E.; Szilvágyi, Gábor; Jakas, Andreja; Laczkó, I.

In: Arkivoc, Vol. 2012, No. 5, 08.06.2012, p. 291-300.

Research output: Contribution to journalArticle

@article{9ab4b590c8e946b2be1733ff5b383ab2,
title = "Structure analysis of proteins, peptides and metal complexes by vibrational circular dichroism",
abstract = "There are two principal forms of vibrational optical activity (VOA), an IR form referred to as vibrational circular dichroism (VCD) and Raman form known as Raman optical activity (ROA). This paper reports examples of the application of VCD spectroscopy for the determination of the absolute configuration and conformation of chiral molecules, e.g. cyclic β-lactams. VCD spectroscopy can be applied for the characterization of the conformation of proteins and peptides in solution. VCD based conformational analysis of cyclic peptides is discussed. Examples are the cyclic hexapeptide cyclo ( Pro2-Gly- Pro2-Gly) and cyclic peptides comprising β-omoamino acids (trans -2-aminocyclopentane or trans-2-aminocyclohexane carboxylic acid). Structure analysis by VCD of opiate peptides, glycopeptides, peptidomimetics and chiral transition metal complexes are also discussed.",
keywords = "Characterization of protein conformation, Determination of absolute configuration, Structure analysis of cyclic peptides, VCD of glycopeptides and chiral transition metal complexes, Vibrational circular dichroism (VCD)",
author = "M. Holl{\'o}si and E. Vass and G{\'a}bor Szilv{\'a}gyi and Andreja Jakas and I. Laczk{\'o}",
year = "2012",
month = "6",
day = "8",
doi = "10.3998/ark.5550190.0013.524",
language = "English",
volume = "2012",
pages = "291--300",
journal = "Arkivoc",
issn = "1551-7012",
publisher = "Arkat USA",
number = "5",

}

TY - JOUR

T1 - Structure analysis of proteins, peptides and metal complexes by vibrational circular dichroism

AU - Hollósi, M.

AU - Vass, E.

AU - Szilvágyi, Gábor

AU - Jakas, Andreja

AU - Laczkó, I.

PY - 2012/6/8

Y1 - 2012/6/8

N2 - There are two principal forms of vibrational optical activity (VOA), an IR form referred to as vibrational circular dichroism (VCD) and Raman form known as Raman optical activity (ROA). This paper reports examples of the application of VCD spectroscopy for the determination of the absolute configuration and conformation of chiral molecules, e.g. cyclic β-lactams. VCD spectroscopy can be applied for the characterization of the conformation of proteins and peptides in solution. VCD based conformational analysis of cyclic peptides is discussed. Examples are the cyclic hexapeptide cyclo ( Pro2-Gly- Pro2-Gly) and cyclic peptides comprising β-omoamino acids (trans -2-aminocyclopentane or trans-2-aminocyclohexane carboxylic acid). Structure analysis by VCD of opiate peptides, glycopeptides, peptidomimetics and chiral transition metal complexes are also discussed.

AB - There are two principal forms of vibrational optical activity (VOA), an IR form referred to as vibrational circular dichroism (VCD) and Raman form known as Raman optical activity (ROA). This paper reports examples of the application of VCD spectroscopy for the determination of the absolute configuration and conformation of chiral molecules, e.g. cyclic β-lactams. VCD spectroscopy can be applied for the characterization of the conformation of proteins and peptides in solution. VCD based conformational analysis of cyclic peptides is discussed. Examples are the cyclic hexapeptide cyclo ( Pro2-Gly- Pro2-Gly) and cyclic peptides comprising β-omoamino acids (trans -2-aminocyclopentane or trans-2-aminocyclohexane carboxylic acid). Structure analysis by VCD of opiate peptides, glycopeptides, peptidomimetics and chiral transition metal complexes are also discussed.

KW - Characterization of protein conformation

KW - Determination of absolute configuration

KW - Structure analysis of cyclic peptides

KW - VCD of glycopeptides and chiral transition metal complexes

KW - Vibrational circular dichroism (VCD)

UR - http://www.scopus.com/inward/record.url?scp=84884198925&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884198925&partnerID=8YFLogxK

U2 - 10.3998/ark.5550190.0013.524

DO - 10.3998/ark.5550190.0013.524

M3 - Article

AN - SCOPUS:84884198925

VL - 2012

SP - 291

EP - 300

JO - Arkivoc

JF - Arkivoc

SN - 1551-7012

IS - 5

ER -