Structural variability of endotoxins from R-type isogenic mutants of Shigella sonnei

Anikõ Kilár, Ãgnes Dörnyei, Annamária Bui, Zoltán Szabõ, Béla Kocsis, Ferenc Kilár

Research output: Contribution to journalArticle

14 Citations (Scopus)

Abstract

The structural variations in the rough-type endotoxins [lipopolysaccharides (LPSs)] of Shigella sonnei mutant strains (S. sonnei phase II-4303, R41, 562H and 4350) were investigated by Matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF-MS) and tandem MS. A series of S. sonnei mutants had previously been the subject of analytical studies on the biosynthesis of heptose components in the core oligosaccharide region of LPSs. This study gives a complete overview on the structures of the full core and lipid A of S. sonnei mutant strains by MS. We found that the LPSs of the isogenic rough mutants were formed in a step-like manner containing 0:1:2:3 heptose in the deep core region of 4350, 562H, R41 and 4303, respectively, and the longest LPS from the mutant S. sonnei 4303 contained also five hexoses. The structural variations in the lipid A moiety and in the oligosaccharide part of the intact LPS were followed by MALDI-TOF-MS/MS. For the dissolution and the ionization of the samples, 2,5-dihydroxybenzoic acid in citric acid solution was applied as matrix. The detailed evaluation of the mass spectra indicates heterogeneity in the lipid part due to the differences in the phosphate and fatty acid composition.

Original languageEnglish
Pages (from-to)61-70
Number of pages10
JournalJournal of Mass Spectrometry
Volume46
Issue number1
DOIs
Publication statusPublished - Jan 2011

Keywords

  • MALDI-TOF-MS
  • MALDI-TOF/TOF-MS
  • Shigella sonnei rough mutant
  • lipopolysaccharide structure

ASJC Scopus subject areas

  • Spectroscopy

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