Structural modifications of lipid vesicles by 8-methoxypsoralen

T. Páli, B. Ebert, L. I. Horváth

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

On prolonged UV-A illumination the ESR spectrum of 16-doxylstearic acid in dimyristoyl phosphatidylcholine vesicles loaded with 8-methoxypsoralen changed dramatically as a second broad component gradually appeared. The composite nature of the spectrum was proven by digital subtraction and the two components were simulated using the modified Bloch equations. From the spectral anisotropy of the motionally averaged three-line component, the molecular order was found to decrease during UV-A illumination, and isotropic rotation of the spin probe was observed well below the phase transition temperature. In parallel an exchange averaged component dominated the central part of the spectrum, indicating the segregation of spin probes into separate domains. The build-up of the composite lineshape was followed by fitting weighted sums of the above two components to spectra recorded after various time intervals. It is concluded that PUVA therapy, which is routinely used against psoriasis, might also induce overlooked damage to the saturated lipid species of biological membranes.

Original languageEnglish
Pages (from-to)359-367
Number of pages9
JournalJournal of Photochemistry and Photobiology, B: Biology
Volume3
Issue number3
DOIs
Publication statusPublished - 1989

Fingerprint

methoxsalen
Methoxsalen
Lighting
Lipids
probes (equipment)
lipids
lighting
PUVA Therapy
psoriasis
Biological membranes
Transition Temperature
Phase Transition
Anisotropy
Composite materials
phase transition
Phosphatidylcholines
Psoriasis
phosphatidylcholines
Superconducting transition temperature
Paramagnetic resonance

Keywords

  • 8-Methoxypsoralen
  • ESR
  • lipid
  • model membrane
  • UV-A.

ASJC Scopus subject areas

  • Plant Science
  • Bioengineering
  • Physical and Theoretical Chemistry

Cite this

Structural modifications of lipid vesicles by 8-methoxypsoralen. / Páli, T.; Ebert, B.; Horváth, L. I.

In: Journal of Photochemistry and Photobiology, B: Biology, Vol. 3, No. 3, 1989, p. 359-367.

Research output: Contribution to journalArticle

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