Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells

S. Damjanovich, G. Vereb, Achim Schaper, A. Jenei, J. Matkó, J. P Pascual Starink, Geoffrey Q. Fox, Donna J. Arndt-Jovin, Thomas M. Jovin

Research output: Contribution to journalArticle

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Abstract

Major histocompatibility complex (MHC) class I antigens in the plasma membranes of human T (HUT-102B2) and B (JY) lymphoma cells were probed by immunochemical reagents using fluorescence, transmission electron, and scanning force microscopies. Fluorescent labels were attached to monoclonal antibodies W6/32 or KE-2 directed against the heavy chain of HLA class I (A, B, C) and L368 or HB28 against the β2-microglobulin light chain. The topological distribution in the nanometer range was studied by photobleaching fluorescence resonance energy transfer (pbFRET) on single cells. A nonrandom codistribution pattern of MHC class I molecules was observed over distances of 2-10 nm. A second, nonrandom, and larger-scale topological organization of the MHC class I antigens was detected by indirect immunogold labeling and imaging by transmission electron microscopy (TEM) and scanning force microscopy (SFM). Although some differences in antigen distribution between the B- and T-cell lines were detected by pbFRET, both cell lines exhibited similar clustering patterns by TEM and SFM. Such defined molecular distributions on the surfaces of cells of the immune system may reflect an underlying specialization of membrane lipid domains and fulfill important functional roles in cell-cell contacts and signal transduction.

Original languageEnglish
Pages (from-to)1122-1126
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume92
Issue number4
DOIs
Publication statusPublished - Feb 14 1995

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Cluster Analysis
Atomic Force Microscopy
Major Histocompatibility Complex
Cell Membrane
Photobleaching
Histocompatibility Antigens Class I
Fluorescence Resonance Energy Transfer
Transmission Electron Microscopy
Cell Line
B-Cell Lymphoma
Membrane Lipids
Electron Scanning Microscopy
Immune System
Signal Transduction
Fluorescence
Monoclonal Antibodies
T-Lymphocytes
Light
Antigens

Keywords

  • antigen presentation
  • atomic force microscopy
  • electron microscopy
  • fluorescence resonance energy transfer
  • scanning force microscopy

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Structural hierarchy in the clustering of HLA class I molecules in the plasma membrane of human lymphoblastoid cells. / Damjanovich, S.; Vereb, G.; Schaper, Achim; Jenei, A.; Matkó, J.; Starink, J. P Pascual; Fox, Geoffrey Q.; Arndt-Jovin, Donna J.; Jovin, Thomas M.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 92, No. 4, 14.02.1995, p. 1122-1126.

Research output: Contribution to journalArticle

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