Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits: Results of a comprehensive survey

András Szilágyi, P. Závodszky

Research output: Contribution to journalArticle

506 Citations (Scopus)

Abstract

Background: Proteins from thermophilic organisms usually show high intrinsic thermal stability but have structures that are very similar to their mesophilic homologues. From previous studies it is difficult to draw general conclusions about the structural features underlying the increased thermal stability of thermophilic proteins. Results: In order to reveal the general evolutionary strategy for changing the heat stability of proteins, a non-redundant data set was compiled comprising all high-quality structures of thermophilic proteins and their mesophilic homologues from the Protein Data Bank. The selection (quality) criteria were met by 64 mesophilic and 29 thermophilic protein subunits, representing 25 protein families. From the atomic coordinates, 13 structural parameters were calculated, compared and evaluated using statistical methods. This study is distinguished from earlier ones by the strict quality control of the structures used and the size of the data set. Conclusions: Different protein families adapt to higher temperatures by different sets of structural devices. Regarding the structural parameters, the only generally observed rule is an increase in the number of ion pairs with increasing growth temperature. Other parameters show just a trend, whereas the number of hydrogen bonds and the polarity of buried surfaces exhibit no clear-cut tendency to change with growth temperature. Proteins from extreme thermophiles are stabilized in different ways to moderately thermophilic ones. The preferences of these two groups are different with regards to the number of ion pairs, the number of cavities, the polarity of exposed surface and the secondary structural composition.

Original languageEnglish
Pages (from-to)493-504
Number of pages12
JournalStructure
Volume8
Issue number5
DOIs
Publication statusPublished - May 1 2000

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Keywords

  • Hyperthermophiles
  • Ion pairs
  • Protein structure
  • Thermophiles
  • Thermostability

ASJC Scopus subject areas

  • Molecular Biology
  • Structural Biology

Cite this

Structural differences between mesophilic, moderately thermophilic and extremely thermophilic protein subunits : Results of a comprehensive survey. / Szilágyi, András; Závodszky, P.

In: Structure, Vol. 8, No. 5, 01.05.2000, p. 493-504.

Research output: Contribution to journalArticle

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