Structural difference between the Ca2+-ATPase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles

L. Dux, Gábor Lelkes, Le Huu Hieu, János Nemcsók

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

1. 1. Structural features were compared in sarcoplasmic reticulum Ca2+-ATPase enzymes from carp (Cyprinus carpio L.) and rabbit muscles. 2. 2. Both membrane preparations contained the 105,000 mol. wt Ca2+ pump protein in high local density. 3. 3. The tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. 4. Addition of vanadate, Ca2+ or lanthanides did not cause two-dimensional Ca2+-ATPase crystal formation, in contrast to the rabbit enzyme, which forms extensive arrays under these conditions. 5. 5. No differences were found in this respect between microsome preparations derived from warm and cold adapted fishes. 6. 6. A different primary sequence as well as a different disposition of the enzyme in the membrane may stand behind the observed dissimilarities.

Original languageEnglish
Pages (from-to)263-270
Number of pages8
JournalComparative Biochemistry and Physiology - B Biochemistry and Molecular Biology
Volume92
Issue number2
DOIs
Publication statusPublished - 1989

Fingerprint

Carps
Calcium-Transporting ATPases
Sarcoplasmic Reticulum
Muscle
Rabbits
Membranes
Muscles
Enzymes
Lanthanoid Series Elements
Peptide Fragments
Vanadates
Microsomes
Fish
Fishes
Pumps
Crystals
Proteins

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Structural difference between the Ca2+-ATPase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles. / Dux, L.; Lelkes, Gábor; Hieu, Le Huu; Nemcsók, János.

In: Comparative Biochemistry and Physiology - B Biochemistry and Molecular Biology, Vol. 92, No. 2, 1989, p. 263-270.

Research output: Contribution to journalArticle

@article{eb093f63735c4ad1b6dc5404ee4f4ac3,
title = "Structural difference between the Ca2+-ATPase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles",
abstract = "1. 1. Structural features were compared in sarcoplasmic reticulum Ca2+-ATPase enzymes from carp (Cyprinus carpio L.) and rabbit muscles. 2. 2. Both membrane preparations contained the 105,000 mol. wt Ca2+ pump protein in high local density. 3. 3. The tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. 4. Addition of vanadate, Ca2+ or lanthanides did not cause two-dimensional Ca2+-ATPase crystal formation, in contrast to the rabbit enzyme, which forms extensive arrays under these conditions. 5. 5. No differences were found in this respect between microsome preparations derived from warm and cold adapted fishes. 6. 6. A different primary sequence as well as a different disposition of the enzyme in the membrane may stand behind the observed dissimilarities.",
author = "L. Dux and G{\'a}bor Lelkes and Hieu, {Le Huu} and J{\'a}nos Nemcs{\'o}k",
year = "1989",
doi = "10.1016/0305-0491(89)90276-9",
language = "English",
volume = "92",
pages = "263--270",
journal = "Comparative biochemistry and physiology. B, Comparative biochemistry",
issn = "1096-4959",
publisher = "Elsevier Inc.",
number = "2",

}

TY - JOUR

T1 - Structural difference between the Ca2+-ATPase enzymes of sarcoplasmic reticulum membrane from rabbit and carp muscles

AU - Dux, L.

AU - Lelkes, Gábor

AU - Hieu, Le Huu

AU - Nemcsók, János

PY - 1989

Y1 - 1989

N2 - 1. 1. Structural features were compared in sarcoplasmic reticulum Ca2+-ATPase enzymes from carp (Cyprinus carpio L.) and rabbit muscles. 2. 2. Both membrane preparations contained the 105,000 mol. wt Ca2+ pump protein in high local density. 3. 3. The tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. 4. Addition of vanadate, Ca2+ or lanthanides did not cause two-dimensional Ca2+-ATPase crystal formation, in contrast to the rabbit enzyme, which forms extensive arrays under these conditions. 5. 5. No differences were found in this respect between microsome preparations derived from warm and cold adapted fishes. 6. 6. A different primary sequence as well as a different disposition of the enzyme in the membrane may stand behind the observed dissimilarities.

AB - 1. 1. Structural features were compared in sarcoplasmic reticulum Ca2+-ATPase enzymes from carp (Cyprinus carpio L.) and rabbit muscles. 2. 2. Both membrane preparations contained the 105,000 mol. wt Ca2+ pump protein in high local density. 3. 3. The tryptic cleavage of the carp enzyme gave different peptide fragments from those observed from rabbit enzyme. 4. 4. Addition of vanadate, Ca2+ or lanthanides did not cause two-dimensional Ca2+-ATPase crystal formation, in contrast to the rabbit enzyme, which forms extensive arrays under these conditions. 5. 5. No differences were found in this respect between microsome preparations derived from warm and cold adapted fishes. 6. 6. A different primary sequence as well as a different disposition of the enzyme in the membrane may stand behind the observed dissimilarities.

UR - http://www.scopus.com/inward/record.url?scp=0024488238&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0024488238&partnerID=8YFLogxK

U2 - 10.1016/0305-0491(89)90276-9

DO - 10.1016/0305-0491(89)90276-9

M3 - Article

C2 - 2522383

AN - SCOPUS:0024488238

VL - 92

SP - 263

EP - 270

JO - Comparative biochemistry and physiology. B, Comparative biochemistry

JF - Comparative biochemistry and physiology. B, Comparative biochemistry

SN - 1096-4959

IS - 2

ER -