Structural components of human cytomegalovirus: In situ localization of the major glycoprotein

M. P. Landini, B. Severi, L. Badiali, E. Gönczöl, G. Mirolo

Research output: Contribution to journalArticle

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Abstract

The localization of the major cytomegalovirus glycoprotein, both in the viral particle and in the cell membrane, was studied by means of indirect immunofluorescence and immunoelectron microscopy using a guinea pig anti-glycoprotein hyperimmune serum recently obtained by Gonczol et al. [1986]. By indirect immunofluorescence a slight and uneven positivity was observed on the plasma membrane of unfixed cells starting from 72 h postinfection (p.i.) until the end of our observation time (7 days p.i.). At immunoelectron microscopy the plasma membrane proved positive only where the virus and dense bodies budded through the membrane to form their own envelope. Extracellular viral particles (both viruses and dense bodies) appeared very strongly labeled on the external surface.

Original languageEnglish
Pages (from-to)154-160
Number of pages7
JournalIntervirology
Volume27
Issue number3
Publication statusPublished - 1987

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Cytomegalovirus
Glycoproteins
Immunoelectron Microscopy
Cell Membrane
Indirect Fluorescent Antibody Technique
Virion
Viruses
Fluorescence Microscopy
Guinea Pigs
Observation
Membranes
Serum

ASJC Scopus subject areas

  • Virology

Cite this

Landini, M. P., Severi, B., Badiali, L., Gönczöl, E., & Mirolo, G. (1987). Structural components of human cytomegalovirus: In situ localization of the major glycoprotein. Intervirology, 27(3), 154-160.

Structural components of human cytomegalovirus : In situ localization of the major glycoprotein. / Landini, M. P.; Severi, B.; Badiali, L.; Gönczöl, E.; Mirolo, G.

In: Intervirology, Vol. 27, No. 3, 1987, p. 154-160.

Research output: Contribution to journalArticle

Landini, MP, Severi, B, Badiali, L, Gönczöl, E & Mirolo, G 1987, 'Structural components of human cytomegalovirus: In situ localization of the major glycoprotein', Intervirology, vol. 27, no. 3, pp. 154-160.
Landini, M. P. ; Severi, B. ; Badiali, L. ; Gönczöl, E. ; Mirolo, G. / Structural components of human cytomegalovirus : In situ localization of the major glycoprotein. In: Intervirology. 1987 ; Vol. 27, No. 3. pp. 154-160.
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