Structural characterization of the second TSP1-module of human thrombospondin

Emoke Roszmusz, A. Pátthy, M. Trexler, L. Patthy

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

The TSP1-module has been first identified as the type 1 repeat of thrombospondin-1. Members of this extracellular module-family have since been shown to be present in several hundred metazoan proteins as well as in proteins of some protists. Despite the widespread occurrence and biological importance of this module-type, relatively little is known about their three-dimensional structure. To define the structural features of this important module-family, we have expressed the second TSP1-domain of human thrombospondin 1 in Escherichia coli. Amino acid sequencing of proteolytic fragments of the recombinant protein have shown that its disulfide bonds connect the six conserved cysteines in a 1-5, 2-6, 3-4 pattern. Circular dichroism studies on the recombinant protein indicate that the disulfide-bonded TSP1-module consists primarily of distorted β-strands.

Original languageEnglish
Pages (from-to)156-160
Number of pages5
JournalBiochemical and Biophysical Research Communications
Volume296
Issue number1
DOIs
Publication statusPublished - 2002

Fingerprint

Thrombospondin 1
Thrombospondins
Recombinant Proteins
Disulfides
Protein Sequence Analysis
Circular Dichroism
Escherichia coli
Cysteine
Proteins
Amino Acids

Keywords

  • Circular dichroism spectroscopy
  • Disulfide connectivity
  • Midkine
  • Pleiotrophin
  • Properdin
  • Thrombospondin

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

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AU - Pátthy, A.

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