Structural characterization of flavonoid glycosides by multi-stage mass spectrometry

Viktoria Vukics, András Guttman

Research output: Contribution to journalArticle

182 Citations (Scopus)


Flavonoids are secondary plant metabolites of great structural variety and high medicinal significance. The search for new chemical entities and the quality control of flavonoid containing natural products require easy-to-use but reliable and robust analytical methodologies. For structural elucidation of flavonoids and their glycosides, nuclear magnetic resonance (NMR) and mass spectroscopy (MS) are the generally used techniques. In phytochemical analyses, however, high amounts of flavonoids are difficult to isolate for NMR, thus low sample volume requiring MS based methods are emerging. This review summarizes and compares currently available methods for structural elucidation of flavonoids by LC-MS and LC-MSn, and focuses on the identification options of unknown flavonoid glycosides in complex samples (e.g., plant extracts) with the emphasis on the differentiation of isomeric compounds.

Original languageEnglish
Pages (from-to)1-16
Number of pages16
JournalMass Spectrometry Reviews
Issue number1
Publication statusPublished - Jan 1 2010



  • Fiavonoid c-glycosides
  • Flavonoid o-glycosides
  • Isomers
  • Structural elucidation

ASJC Scopus subject areas

  • Analytical Chemistry
  • Condensed Matter Physics
  • Biochemistry, Genetics and Molecular Biology(all)
  • Spectroscopy

Cite this