Structural and functional features and significance of the physical linkage between ER and mitochondria

György Csordás, Christian Renken, P. Várnai, Ludivine Walter, David Weaver, Karolyn F. Buttle, Tamás Balla, Carmen A. Mannella, György Hajnóczky

Research output: Contribution to journalArticle

705 Citations (Scopus)

Abstract

The role of mitochondria in cell metabolism and survival is controlled by calcium signals that are commonly transmitted at the close associations between mitochondria and endoplasmic reticulum (ER). However, the physical linkage of the ER-mitochondria interface and its relevance for cell function remains elusive. We show by electron tomography that ER and mitochondria are adjoined by tethers that are ∼10 nm at the smooth ER and ∼25 nm at the rough ER. Limited proteolysis separates ER from mitochondria, whereas expression of a short "synthetic linker" (2+ overloading and ensuing permeability transition. These results reveal an unexpected dependence of cell function and survival on the maintenance of proper spacing between the ER and mitochondria.

Original languageEnglish
Pages (from-to)915-921
Number of pages7
JournalJournal of Cell Biology
Volume174
Issue number7
DOIs
Publication statusPublished - Sep 25 2006

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Endoplasmic Reticulum
Mitochondria
Cell Survival
Electron Microscope Tomography
Smooth Endoplasmic Reticulum
Rough Endoplasmic Reticulum
Proteolysis
Permeability
Maintenance
Calcium

ASJC Scopus subject areas

  • Cell Biology

Cite this

Structural and functional features and significance of the physical linkage between ER and mitochondria. / Csordás, György; Renken, Christian; Várnai, P.; Walter, Ludivine; Weaver, David; Buttle, Karolyn F.; Balla, Tamás; Mannella, Carmen A.; Hajnóczky, György.

In: Journal of Cell Biology, Vol. 174, No. 7, 25.09.2006, p. 915-921.

Research output: Contribution to journalArticle

Csordás, G, Renken, C, Várnai, P, Walter, L, Weaver, D, Buttle, KF, Balla, T, Mannella, CA & Hajnóczky, G 2006, 'Structural and functional features and significance of the physical linkage between ER and mitochondria', Journal of Cell Biology, vol. 174, no. 7, pp. 915-921. https://doi.org/10.1083/jcb.200604016
Csordás, György ; Renken, Christian ; Várnai, P. ; Walter, Ludivine ; Weaver, David ; Buttle, Karolyn F. ; Balla, Tamás ; Mannella, Carmen A. ; Hajnóczky, György. / Structural and functional features and significance of the physical linkage between ER and mitochondria. In: Journal of Cell Biology. 2006 ; Vol. 174, No. 7. pp. 915-921.
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