Structural and functional changes in the photosynthetic apparatus of Chlamydomonas reinhardtii during nitrogen deprivation and replenishment

Preininger, A. Kósa, Z. S. Lőrincz, P. Nyitrai, J. Simon, B. Böddi, Keresztes, I. Gyurján

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Abstract

Nitrogen is an essential factor for normal plant and algal development. As a component of nucleic acids, proteins, and chlorophyll (Chl) molecules, it has a crucial role in the organization of a functioning photosynthetic apparatus. Our aim was to study the effects of nitrogen starvation in cultures of the unicellular green alga, Chlamydomonas reinhardtii, maintained on nitrogen-free, and then on nitrogen-containing medium. During the three-week-long degreening process, considerable changes were observed in the Chl content, the ratio of Chl-protein complexes, and photosynthetic activity of the cultures as well as in the ultrastructure of single chloroplasts. The regreening process was much faster then the degradation; total greening of the cells occurred within four days. The rate of regeneration depended on the nitrogen content. At least 50% of the normal nitrogen content of Tris-Acetate-Phosphate (TAP) medium was required in the medium for the complete regreening of the cells and regeneration of chloroplasts.

Original languageEnglish
Pages (from-to)369-377
Number of pages9
JournalPhotosynthetica
Volume53
Issue number3
DOIs
Publication statusPublished - Sep 27 2015

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Keywords

  • 77K fluorescence
  • O evolution
  • electron microscopy
  • nitrogen starvation

ASJC Scopus subject areas

  • Physiology
  • Plant Science

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