Structural analysis of a rhamnoarabinogalactan and arabinogalactans with immuno-stimulating activity from Calendula officinalis

Jadranka Varljen, Andras Lipták, Hildebert Wagner

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From the flowers of Calendula officinalis, three homogeneous polysaccharides were isolated by ethanol fractionation and gel filtration. Sugar and methylation analysis showed that all three polysaccharides contain a (1→3)-linked β-d-galactan backbone with branching points at C-6. The side chains, are composed of short α-Araf(1→3)-Araf, α-l-Rhap-(1→3)-Araf or simple α-l-Araf units. Further characterization of the polysaccharides was performed by periodate oxidation and Smith degradation, acid hydrolysis and various 13C NMR spectroscopic methods. The polysaccharides showed immunostimulating activity in several in vitro immunological test systems.

Original languageEnglish
Pages (from-to)2379-2383
Number of pages5
Issue number9
Publication statusPublished - 1989



  • Asteraceae
  • Calendula officinalis
  • arabinogalactans
  • immunological activity.
  • plant polysaccharides
  • rhamnoarabinogalactan
  • structural analysis

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Plant Science
  • Horticulture

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