Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance

Istvan Bock, Eszter Losonczi, Solomon Mamo, Zsuzsanna Polgar, Andrea Hamos, A. Dinnyés, Csaba Pribenszky

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Sublethal high hydrostatic pressure (HHP) treatment of cells was reported to enhance stress tolerance and to increase post-thawing survival after cryopreservation in mouse, swine and cattle. The goal of this study was to define if HHP stress tolerance depends on the embryos' stage of development and culture conditions, to describe long term in vivo effects and transcriptional alterations of selected stress related genes. Studies showed that impacts greater than 60 MPa caused blastomere and membrane injuries to the two-cell stage embryos, while even 80 MPa was well tolerated by blastocysts. HHP treatment caused significant upregulation of Azin 1, Sod2 and Gadd45g genes, detected by RT-qPCR. The transfer of HHP treated blastocysts revealed normal in vivo development and reproductive function in a two generation study. The cell type and the embryos' development stage shall be taken into account when optimizing sublethal HHP stress treatment protocol of different cells.

Original languageEnglish
Pages (from-to)401-412
Number of pages12
JournalCryo-Letters
Volume31
Issue number5
Publication statusPublished - Sep 2010

Fingerprint

Hydrostatic Pressure
stress tolerance
embryo (animal)
Embryonic Structures
mice
high pressure treatment
blastocyst
Blastocyst
Embryonic Development
cells
blastomeres
Blastomeres
thawing
cryopreservation
Cryopreservation
Clinical Protocols
embryogenesis
genes
Genes
Up-Regulation

Keywords

  • Gene expression
  • High hydrostatic pressure
  • Morphology
  • Mouse embryo
  • Stress tolerance

ASJC Scopus subject areas

  • Agronomy and Crop Science
  • Anatomy

Cite this

Bock, I., Losonczi, E., Mamo, S., Polgar, Z., Hamos, A., Dinnyés, A., & Pribenszky, C. (2010). Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance. Cryo-Letters, 31(5), 401-412.

Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance. / Bock, Istvan; Losonczi, Eszter; Mamo, Solomon; Polgar, Zsuzsanna; Hamos, Andrea; Dinnyés, A.; Pribenszky, Csaba.

In: Cryo-Letters, Vol. 31, No. 5, 09.2010, p. 401-412.

Research output: Contribution to journalArticle

Bock, I, Losonczi, E, Mamo, S, Polgar, Z, Hamos, A, Dinnyés, A & Pribenszky, C 2010, 'Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance', Cryo-Letters, vol. 31, no. 5, pp. 401-412.
Bock, Istvan ; Losonczi, Eszter ; Mamo, Solomon ; Polgar, Zsuzsanna ; Hamos, Andrea ; Dinnyés, A. ; Pribenszky, Csaba. / Stress tolerance and transcriptional response in mouse embryos treated with high hydrostatic pressure to enhance cryotolerance. In: Cryo-Letters. 2010 ; Vol. 31, No. 5. pp. 401-412.
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