Stress preconditioning of semen before cryopreservation improves fertility and increases the number of offspring born: A prospective randomised study using a porcine model

A. Horváth, O. Szenci, K. Nagy, L. Végh, Cs Pribenszky

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The aim of the present study was to investigate the effect of applying sublethal stress treatment at room temperature, before cryopreservation (hydrostatic pressure (HP): 40MPa, 80min) of 34 boar ejaculate samples, on post-thawed motility and sow fertility. Sows (n≤102) were randomly allocated into equal groups inseminated with HP-treated or untreated frozen-thawed semen. Sows were inseminated twice, 10h apart, with 6×109 spermatozoa per dose without oestrus synchronisation. Rates of non-return of oestrus and pregnancy, and total numbers of piglets and live piglets were significantly higher (P0.05), improvement in the farrowing rate in the HP-treated group. Although the number of live piglets per litter decreased approximately 15% in both groups by 42 days after farrowing, but this remained significantly higher in the HP-treated group. Although total and progressive sperm motility were significantly (P0.05) in these parameters between pregnant and non-pregnant sows in either group; thus motility can indicate, but not predict, improved fertility. In conclusion, HP treatment, with sperm cryopreservation, increases in vitro sperm motility and improves reproductive performance without adversely affecting the health of the piglets.

Original languageEnglish
Pages (from-to)475-481
Number of pages7
JournalReproduction, Fertility and Development
Volume28
Issue number4
DOIs
Publication statusPublished - 2016

Fingerprint

Hydrostatic Pressure
Cryopreservation
Semen
cryopreservation
Fertility
sows
piglets
semen
Swine
Prospective Studies
swine
sperm motility
Sperm Motility
spermatozoa
Spermatozoa
farrowing rate
pressure treatment
estrus synchronization
Estrus Synchronization
sublethal effects

Keywords

  • boar
  • deep freezing
  • hydrostatic pressure
  • post-thaw motility.

ASJC Scopus subject areas

  • Animal Science and Zoology
  • Reproductive Medicine
  • Developmental Biology
  • Endocrinology
  • Genetics
  • Molecular Biology
  • Biotechnology

Cite this

@article{363a50a347db4f5e8bae339289cde0c9,
title = "Stress preconditioning of semen before cryopreservation improves fertility and increases the number of offspring born: A prospective randomised study using a porcine model",
abstract = "The aim of the present study was to investigate the effect of applying sublethal stress treatment at room temperature, before cryopreservation (hydrostatic pressure (HP): 40MPa, 80min) of 34 boar ejaculate samples, on post-thawed motility and sow fertility. Sows (n≤102) were randomly allocated into equal groups inseminated with HP-treated or untreated frozen-thawed semen. Sows were inseminated twice, 10h apart, with 6×109 spermatozoa per dose without oestrus synchronisation. Rates of non-return of oestrus and pregnancy, and total numbers of piglets and live piglets were significantly higher (P0.05), improvement in the farrowing rate in the HP-treated group. Although the number of live piglets per litter decreased approximately 15{\%} in both groups by 42 days after farrowing, but this remained significantly higher in the HP-treated group. Although total and progressive sperm motility were significantly (P0.05) in these parameters between pregnant and non-pregnant sows in either group; thus motility can indicate, but not predict, improved fertility. In conclusion, HP treatment, with sperm cryopreservation, increases in vitro sperm motility and improves reproductive performance without adversely affecting the health of the piglets.",
keywords = "boar, deep freezing, hydrostatic pressure, post-thaw motility.",
author = "A. Horv{\'a}th and O. Szenci and K. Nagy and L. V{\'e}gh and Cs Pribenszky",
year = "2016",
doi = "10.1071/RD14118",
language = "English",
volume = "28",
pages = "475--481",
journal = "Reproduction, Fertility and Development",
issn = "1031-3613",
publisher = "CSIRO",
number = "4",

}

TY - JOUR

T1 - Stress preconditioning of semen before cryopreservation improves fertility and increases the number of offspring born

T2 - A prospective randomised study using a porcine model

AU - Horváth, A.

AU - Szenci, O.

AU - Nagy, K.

AU - Végh, L.

AU - Pribenszky, Cs

PY - 2016

Y1 - 2016

N2 - The aim of the present study was to investigate the effect of applying sublethal stress treatment at room temperature, before cryopreservation (hydrostatic pressure (HP): 40MPa, 80min) of 34 boar ejaculate samples, on post-thawed motility and sow fertility. Sows (n≤102) were randomly allocated into equal groups inseminated with HP-treated or untreated frozen-thawed semen. Sows were inseminated twice, 10h apart, with 6×109 spermatozoa per dose without oestrus synchronisation. Rates of non-return of oestrus and pregnancy, and total numbers of piglets and live piglets were significantly higher (P0.05), improvement in the farrowing rate in the HP-treated group. Although the number of live piglets per litter decreased approximately 15% in both groups by 42 days after farrowing, but this remained significantly higher in the HP-treated group. Although total and progressive sperm motility were significantly (P0.05) in these parameters between pregnant and non-pregnant sows in either group; thus motility can indicate, but not predict, improved fertility. In conclusion, HP treatment, with sperm cryopreservation, increases in vitro sperm motility and improves reproductive performance without adversely affecting the health of the piglets.

AB - The aim of the present study was to investigate the effect of applying sublethal stress treatment at room temperature, before cryopreservation (hydrostatic pressure (HP): 40MPa, 80min) of 34 boar ejaculate samples, on post-thawed motility and sow fertility. Sows (n≤102) were randomly allocated into equal groups inseminated with HP-treated or untreated frozen-thawed semen. Sows were inseminated twice, 10h apart, with 6×109 spermatozoa per dose without oestrus synchronisation. Rates of non-return of oestrus and pregnancy, and total numbers of piglets and live piglets were significantly higher (P0.05), improvement in the farrowing rate in the HP-treated group. Although the number of live piglets per litter decreased approximately 15% in both groups by 42 days after farrowing, but this remained significantly higher in the HP-treated group. Although total and progressive sperm motility were significantly (P0.05) in these parameters between pregnant and non-pregnant sows in either group; thus motility can indicate, but not predict, improved fertility. In conclusion, HP treatment, with sperm cryopreservation, increases in vitro sperm motility and improves reproductive performance without adversely affecting the health of the piglets.

KW - boar

KW - deep freezing

KW - hydrostatic pressure

KW - post-thaw motility.

UR - http://www.scopus.com/inward/record.url?scp=84959349343&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84959349343&partnerID=8YFLogxK

U2 - 10.1071/RD14118

DO - 10.1071/RD14118

M3 - Article

C2 - 25167844

AN - SCOPUS:84959349343

VL - 28

SP - 475

EP - 481

JO - Reproduction, Fertility and Development

JF - Reproduction, Fertility and Development

SN - 1031-3613

IS - 4

ER -