Stress-induced changes in the free amino acid composition in transgenic soybean plants having increased proline content

L. Simon-Sarkadi, G. Kocsy, Á Várhegyi, G. Galiba, J. A. De Ronde

Research output: Contribution to journalArticle

37 Citations (Scopus)

Abstract

Following drought stress at supraoptimal temperature the increase in proline (Pro) content in transgenic (T) soybean [Glycine max (L.) Merr. cv. Ibis] plants overexpressing the gene coding for the last enzyme of Pro biosynthesis, L-Δ1-pyrroline-5-carboxylate reductase, was much greater than in wild type (W) plants (105-fold versus 19-fold after 7 d). Under control conditions arginine accounted for nearly 60 % of the total free amino acid content. After stress treatment the content of Pro was more than 50 % in both T and W genotypes, and at the end of recovery the γ-aminobutyrate content reached 27 and 53 % in the W and T plants, respectively. Without stress treatment there was only a 2-fold difference between T and W in the tyrosine content. However, during the stress period and the subsequent recovery a similar difference was found for many amino acids. The present results indicate that manipulating of the content of a single amino acid influences the whole free amino acid composition in soybean.

Original languageEnglish
Pages (from-to)793-796
Number of pages4
JournalBiologia Plantarum
Volume50
Issue number4
DOIs
Publication statusPublished - Dec 2006

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amino acid composition
free amino acids
proline
genetically modified organisms
soybeans
amino acids
arginine
tyrosine
Glycine max
water stress
biosynthesis
genotype
enzymes
temperature
genes

Keywords

  • Drought
  • Glycine max
  • Heat

ASJC Scopus subject areas

  • Plant Science

Cite this

Stress-induced changes in the free amino acid composition in transgenic soybean plants having increased proline content. / Simon-Sarkadi, L.; Kocsy, G.; Várhegyi, Á; Galiba, G.; De Ronde, J. A.

In: Biologia Plantarum, Vol. 50, No. 4, 12.2006, p. 793-796.

Research output: Contribution to journalArticle

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AU - De Ronde, J. A.

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