Strain-specific SCAR markers for the detection of Trichoderma harzianum AS12-2, a biological control agent against Rhizocto nia solani, the causal agent of rice sheath blight

S. Naeimi, S. Kocsubé, Zsuzsanna Antal, S. Okhovvat, M. Javan-Nikkhah, C. Vágvölgyi, L. Kredics

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

In order to identify a specific marker for T. harzianum AS12-2, a strain capable of controlling rice sheath blight caused by Rhizoctonia solani, UP-PCR was performed using five universal primers (UP) both separately and in pairwise combinations. The application of two UP primers resulted in the amplification of unique fragments from the genomic DNA of T. harzianum AS12-2, clearly distinguishing it from other Trichoderma strains. The unique fragments had no significant sequence homology with any other known sequence available in databases. Based on the sequences of the unique fragments, 14 oligonucleotide primers were designed. Two primer sets amplified a fragment of expected size from the DNA of strain T. harzianum AS12-2 but not from any other examined strains belonging to T. harzianum, to other Trichoderma species assayed, or to other common fungi present in paddy fields of Mazandaran province, Iran. In conclusion, SCAR (sequence characterized amplified regions) markers were successfully identified and rapid, reliable tools were provided for the detection of an effective biocontrol Trichoderma strain, which can facilitate studies of its population dynamics and establishment after release into the natural environment.

Original languageEnglish
Pages (from-to)73-84
Number of pages12
JournalActa biologica Hungarica
Volume62
Issue number1
DOIs
Publication statusPublished - Mar 1 2011

Keywords

  • Rhizoctonia solani
  • SCAR markers
  • Trichoderma harzianum
  • biological control
  • rice sheath blight

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Environmental Science(all)
  • Neurology

Fingerprint Dive into the research topics of 'Strain-specific SCAR markers for the detection of Trichoderma harzianum AS12-2, a biological control agent against Rhizocto nia solani, the causal agent of rice sheath blight'. Together they form a unique fingerprint.

  • Cite this