Strain-dependent variations in kinetic properties of mesencephalic tyrosine hydroxylase

I. Sziraki, M. Sasvári, P. Kabai, G. Kobor, I. Vadasz, A. Lajtha, C. Vadasz

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Kinetic properties of mesencephalic tyrosine hydroxylase (TH) were investigated in three highly inbred mouse strains: BALB/cJ, C57BL/6ByJ and CXBI/ByJ. A significant (P <0.005) genetic variation was found among the apparent maximal velocities of the enzyme reaction (V(max)) without any significant differences in the values of apparent Michaelis-Menten constants (K(m)). Moreover, a biphasic pattern was found when TH activity was measured as the function of the cofactor (6-methyl-5,6,7,8-tetrahydropterin) concentration, indicating the presence of both the phosphorylated (high-affinity, HA) form and the non-phosphorylated (low-affinity, LA) form of TH. Again, there was no significant genetic source of differences among the K(m) values. However, the V(max) of the LA form was significantly higher in BALB/cJ than in the other two strains, whereas C57BL/6ByJ and CXBI/ByJ differed in the V(max) values of the HA form of TH.

Original languageEnglish
Pages (from-to)177-185
Number of pages9
JournalBiogenic Amines
Volume6
Issue number2
Publication statusPublished - 1989

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Tyrosine 3-Monooxygenase
Inbred Strains Mice
Enzymes

ASJC Scopus subject areas

  • Neuroscience(all)
  • Pharmacology

Cite this

Sziraki, I., Sasvári, M., Kabai, P., Kobor, G., Vadasz, I., Lajtha, A., & Vadasz, C. (1989). Strain-dependent variations in kinetic properties of mesencephalic tyrosine hydroxylase. Biogenic Amines, 6(2), 177-185.

Strain-dependent variations in kinetic properties of mesencephalic tyrosine hydroxylase. / Sziraki, I.; Sasvári, M.; Kabai, P.; Kobor, G.; Vadasz, I.; Lajtha, A.; Vadasz, C.

In: Biogenic Amines, Vol. 6, No. 2, 1989, p. 177-185.

Research output: Contribution to journalArticle

Sziraki, I, Sasvári, M, Kabai, P, Kobor, G, Vadasz, I, Lajtha, A & Vadasz, C 1989, 'Strain-dependent variations in kinetic properties of mesencephalic tyrosine hydroxylase', Biogenic Amines, vol. 6, no. 2, pp. 177-185.
Sziraki, I. ; Sasvári, M. ; Kabai, P. ; Kobor, G. ; Vadasz, I. ; Lajtha, A. ; Vadasz, C. / Strain-dependent variations in kinetic properties of mesencephalic tyrosine hydroxylase. In: Biogenic Amines. 1989 ; Vol. 6, No. 2. pp. 177-185.
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AU - Sasvári, M.

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AU - Vadasz, I.

AU - Lajtha, A.

AU - Vadasz, C.

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