Stimulation of deoxycytidine kinase results in prolonged maintenance of the enzyme activity

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Abstract

A number of genotoxic and antiproliferative agents such as 2-chlorodeoxyadenosine (Cladribine; CdA) and aphidicolin (APC) have been shown to stimulate the activity of deoxycytidine kinase, the main deoxynucleoside salvage enzyme in lymphocytes. Here we show that enzyme activation could be prevented by treating cells with the membrane-permeant calcium chelator BAPTA-AM. Long-term incubations demonstrated that CdA and APC not only stimulated but also sustained deoxycytidine kinase activity in the cellular context, as compared to the control and BAPTA-AM treated enzyme activities.

Original languageEnglish
Pages (from-to)1357-1361
Number of pages5
JournalNucleosides, Nucleotides and Nucleic Acids
Volume23
Issue number8-9
DOIs
Publication statusPublished - Dec 23 2004

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Keywords

  • 2-Chlorodeoxyadenosine
  • BAPTA-AM
  • Calcium chelation
  • Deoxycytidine kinase
  • Enzyme activation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Medicine
  • Genetics

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